阅读说明：本技术 一种燕麦ace抑制肽的制备方法及其产品 (Preparation method of oat ACE inhibitory peptide and product thereof ) 是由 任清 孙静 王晶淼 张莎莎 邓巍 左桥 于 2019-09-30 设计创作，主要内容包括：本发明涉及燕麦多肽的制备技术领域,具体为一种燕麦ACE抑制肽的制备方法及其产品,该方法是将燕麦麸加入去离子水,调节PH至9.5,超声波生化仪中,浸提,再离心；取上清液调节PH至4.0,离心,慢慢倒掉上清液,保留燕麦蛋白沉淀,并将燕麦蛋白沉淀冷冻干燥存储。通过本方法制得的ACE抑制肽的相对分子量在800Da以下,能够制得ACE抑制性达到86.34％的燕麦ACE抑制肽,同时也避免了使用蠕动泵和超滤膜而导致Alcalase酶活损失的问题,提高了燕麦ACE抑制肽的生产效率。(The invention relates to the technical field of preparation of oat polypeptide, in particular to a preparation method of oat ACE inhibitory peptide and a product thereof, wherein the method comprises the steps of adding oat bran into deionized water, adjusting pH to 9.5, leaching in an ultrasonic biochemical instrument, and centrifuging; and (3) taking the supernatant, adjusting the pH value to 4.0, centrifuging, slowly pouring out the supernatant, retaining the oat protein precipitate, and freeze-drying and storing the oat protein precipitate. The relative molecular weight of the ACE inhibitory peptide prepared by the method is below 800Da, the oat ACE inhibitory peptide with the ACE inhibitory property of 86.34% can be prepared, the problem of Alcalase enzyme activity loss caused by a peristaltic pump and an ultrafiltration membrane is avoided, and the production efficiency of the oat ACE inhibitory peptide is improved.)

1. A preparation method of oat ACE inhibitory peptide is characterized by comprising the following steps:

extracting oat protein: adding deionized water into oat bran according to the feed-liquid ratio of 1:18g/ml, adjusting the pH to 9.5, leaching for 60min in an ultrasonic biochemical instrument, and centrifuging for 10min at the speed of 5000 r/min; taking the supernatant, adjusting the pH value to 4.0, centrifuging at 4000r/min for 15min, slowly pouring out the supernatant, keeping the oat protein precipitate, and freeze-drying and storing the oat protein precipitate;

preparation of ACE inhibitory peptides: dissolving oat protein in deionized water to obtain protein solution with mass concentration of 5%, adding protease, placing in a constant temperature enzyme reactor at 60-100 deg.C, and performing enzymolysis for 1-4 hr by using protease selected from one or more of Alcalase, Trypsin, Pepsin, Pancreatin, Neutrase, Flavourzyme and Protamex.

2. The method for preparing oat ACE inhibitory peptide according to claim 1, wherein the method comprises the following steps: in the preparation of ACE inhibiting peptides, the protease used was Alcalase.

3. The method for preparing oat ACE inhibitory peptide according to claim 1, wherein the method comprises the following steps: in the preparation of ACE inhibiting peptides, the temperature of the isothermal enzyme reactor was set at 85 ℃.

4. The method for preparing oat ACE inhibitory peptide according to claim 1, wherein the method comprises the following steps: in the preparation of ACE inhibitory peptide, the enzymolysis time is 3 h.

5. The method for preparing oat ACE inhibitory peptide according to claim 1, wherein the method comprises the following steps: in the preparation of the ACE inhibitory peptide, decolorization treatment is also included;

and (3) decoloring treatment: when an enzyme reactor carries out enzymolysis on the oat protein, adding activated carbon particles for decoloring for 1h, stirring the enzyme reactor at a speed of 100r/min by using a stirrer during decoloring, and carrying out suction filtration on enzyme liquid by using a filter membrane, wherein the cut-off molecular weight of the filter membrane is 400-plus-500 Da.

6. A product of oat ACE inhibitory peptide, which is characterized by: mixing the oat ACE inhibitory peptide prepared by the method of any one of claims 1-5 with dendrobii polysaccharide, wherein the mass ratio of the ACE inhibitory peptide to the dendrobii polysaccharide is 1:1, and adding the mixture of the oat ACE inhibitory peptide and dendrobii polysaccharide into a vitamin C tablet according to the mass ratio of 1: 5-20.

7. The product of oat ACE inhibitory peptide of claim 6, wherein: the ACE inhibitory peptide, the dendrobe polysaccharide and the vitamin C are mixed according to the mass ratio of 1:1:5-20, then oat bran is added for mixing, and the mixture is pressed into a sheet product through a tablet press.

Technical Field

The invention relates to the technical field of preparation of oat polypeptide, in particular to a preparation method of oat ACE inhibitory peptide and a product thereof.

Background

Hypertension is a main cause of complications such as coronary heart disease, myocardial infarction, cerebral apoplexy, renal failure and the like, is liked to be the first killer of human health, Angiotensin Converting Enzyme (ACE) is a key enzyme for raising blood pressure, and the aim of preventing and treating hypertension can be achieved by inhibiting the activity of ACE. At present, ACE inhibitor medicines are mainly used for treating hypertension, and are mainly synthesized chemically, so that although the ACE inhibitor medicines have high activity and specificity, serious toxic and side effects can be generated in the taking process. Therefore, safe and non-toxic side effect ACE inhibitor medicines become the research direction of novel antihypertensive medicines, and food source ACE inhibitory peptide just meets the requirement, thereby providing a new approach for developing novel antihypertensive medicines.

The oat is a special crop with multiple purposes of grain, menses, feed and medicine, has high nutrition and health care functions, is rich in soluble dietary fiber, vitamin mineral substances and antioxidant ingredients, contains protein in naked oat at the head of the crop, and has the protein content 1.6-2.3 times of that of wheat flour and soybean protein.

Disclosure of Invention

This section is for the purpose of summarizing some aspects of embodiments of the invention and to briefly introduce some preferred embodiments. In this section, as well as in the abstract and the title of the invention of this application, simplifications or omissions may be made to avoid obscuring the purpose of the section, the abstract and the title, and such simplifications or omissions are not intended to limit the scope of the invention.

The present invention has been made in view of the above problems.

In order to solve the technical problems, the invention provides the following technical scheme:

the invention relates to a preparation method of oat ACE inhibitory peptide, which comprises the following steps:

extracting oat protein: adding deionized water into oat bran according to the feed-liquid ratio of 1:18g/ml, adjusting the pH to 9.5, leaching for 60min in an ultrasonic biochemical instrument, and centrifuging for 10min at the speed of 5000 r/min; taking the supernatant, adjusting the pH value to 4.0, centrifuging at 4000r/min for 15min, slowly pouring out the supernatant, keeping the oat protein precipitate, and freeze-drying and storing the oat protein precipitate;

preparation of ACE inhibitory peptides: dissolving oat protein in deionized water to obtain protein solution with mass concentration of 5%, adding protease, placing in a constant temperature enzyme reactor at 60-100 deg.C, and performing enzymolysis for 1-4 hr by using protease selected from one or more of Alcalase, Trypsin, Pepsin, Pancreatin, Neutrase, Flavourzyme and Protamex.

Preferably, in the preparation of ACE inhibiting peptides, the protease used is Alcalase.

Preferably, in the preparation of ACE inhibiting peptides, the temperature of the isothermal enzyme reactor is set at 85 ℃.

Preferably, in the preparation of the ACE inhibitory peptide, the enzymolysis time is 3 h.

Preferably, in the preparation of the ACE inhibitory peptide, decolorization treatment is also included;

and (3) decoloring treatment: when an enzyme reactor carries out enzymolysis on the oat protein, adding activated carbon particles for decoloring for 1h, stirring the enzyme reactor at a speed of 100r/min by using a stirrer during decoloring, and carrying out suction filtration on enzyme liquid by using a filter membrane, wherein the cut-off molecular weight of the filter membrane is 400-plus-500 Da.

A product of oat ACE inhibitory peptide is prepared by mixing oat ACE inhibitory peptide and dendrobe polysaccharide, wherein the mass ratio of the ACE inhibitory peptide to the dendrobe polysaccharide is 1:1, and the mixture of the oat ACE inhibitory peptide and dendrobe polysaccharide is added into vitamin C tablets according to the mass ratio of 1: 5-20.

Preferably, the ACE inhibitory peptide, the dendrobe polysaccharide and the vitamin C are mixed according to the mass ratio of 1:1:5-20, oat bran is added for mixing, and the mixture is pressed into a tablet product through a tablet press.

Compared with the prior art: the preparation method of the oat ACE inhibitory peptide comprises the steps of adding deionized water into oat bran according to the material-liquid ratio of 1:18g/ml, adjusting the pH value to 9.5, leaching for 60min in an ultrasonic biochemical instrument, and centrifuging for 10min at the speed of 5000 r/min; taking the supernatant, adjusting the pH value to 4.0, centrifuging at 4000r/min for 15min, slowly pouring out the supernatant, keeping the oat protein precipitate, and freeze-drying and storing the oat protein precipitate; carrying out enzymolysis, wherein the relative molecular weight of the ACE inhibitory peptide prepared by the method is below 800Da, so that oat ACE inhibitory peptide with ACE inhibitory property reaching 86.34% can be prepared, meanwhile, the problem of Alcalase enzyme activity loss caused by using a peristaltic pump and an ultrafiltration membrane is avoided, and the production efficiency of the oat ACE inhibitory peptide is improved; a product of oat ACE inhibitory peptide is prepared by mixing oat ACE inhibitory peptide and dendrobe polysaccharide, wherein the mass ratio of the ACE inhibitory peptide to the dendrobe polysaccharide is 1:1, and then adding the mixture of the oat ACE inhibitory peptide and dendrobe polysaccharide into a vitamin C tablet according to the mass ratio of 1: 15; the oat ACE inhibitory peptide is used for reducing blood pressure, the antioxidant activity of the dendrobium polysaccharide and vitamin C are used for repairing blood vessels, and hypertension and cerebral thrombosis can be effectively treated.

DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION

The following will clearly and completely describe the technical solutions in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The methods of the following examples are conventional unless otherwise specified.

The percentages given in the following examples are by weight unless otherwise specified.

Materials used in the following examples: oat bran is provided by Shanxi Datong oat processing plant; ACE and hippuric acid-histidine-leucine were purchased from Sigma; the protease is purchased from Novo company, and all the reagents are analytically pure;

the apparatus used in the following examples: the HSG electric heating constant temperature water bath is provided by Shanghai instruments (group) for marketing company; the CS501 type super thermostat is provided by Chongqing experiment equipment factories; the 90-2 type constant temperature magnetic stirrer is manufactured by Shanghai Yanglong Biochemical apparatus factories; UV-2800 model UV-visible spectrophotometer by Yonick instruments Inc.