阅读说明：本技术 用于确定生物分子之间的相互作用水平的方法 (Method for determining the level of the interaction between biomolecule ) 是由 奥拉·索德伯格 于 2018-02-09 设计创作，主要内容包括：本发明涉及用于确定样品中的生物分子诸如蛋白质之间的相互作用水平的方法,其包括提供第一和第二携带信息(IC)的寡核苷酸,其中第一和第二IC寡核苷酸共价地或非共价地附接至具有与第一和第二生物分子结合的能力的第一和第二亲和试剂诸如抗体,其中第一和第二IC寡核苷酸各自包含与另一个寡核苷酸的一部分互补的至少一个单链链段,从而在第一和第二IC寡核苷酸中的至少一个中的至少一个单链链段与另一个寡核苷酸的其互补部分杂交后,能够在单细胞水平或单分子水平上测量样品中相互作用的第一和第二生物分子与非相互作用的第一和第二生物分子的相对比例。此外,本发明涉及包含实施本发明的方法必需的寡核苷酸和试剂的试剂盒。(The present invention relates to the methods for determining the interaction level between such as protein of the biomolecule in sample, it includes providing the first and second oligonucleotides for carrying information (IC), wherein the first and second IC oligonucleotides are covalently or non-covalently attached to the first and second affinity reagents such as antibody with the ability in conjunction with the first and second biomolecule, wherein the first and second IC oligonucleotides respectively contain a part of at least one complementary single-stranded segment with another oligonucleotides, to after the single-stranded segment of at least one of at least one of first and second IC oligonucleotides hybridizes with its complementary portion of another oligonucleotides, the phase of the first and second biomolecule and non-interacting first and second biomolecule that interact in sample can be measured on individual cell level or single molecules level Comparative example.In addition, the present invention relates to the kits comprising implementing oligonucleotides and reagent necessary to method of the invention.)