阅读说明：本技术 一种原生药粉的微生物检测方法及其灭菌方法 (Microorganism detection method and sterilization method for crude drug powder ) 是由 周映佑 滕钰 黄婕 薛咏兰 徐洪 罗曼 王俊 严利娟 孙宜春 李慧馨 于 2020-05-06 设计创作，主要内容包括：本发明提供了一种原生药粉的微生物检测方法,属于中医药领域,具体方法为将供试品直接接种至培养基中培养；取上述培养液至RV沙门增菌液体培养基中再次培养；将该培养物接种于木糖赖氨酸脱氧胆酸盐琼脂培养基培养得疑似菌落。用接种针挑选疑似菌落于三糖铁琼脂培养基高层斜面上进行斜面和高层穿刺接种培养,从而进行根据结果判断微生物的情况,本发明方法简单易行,解决了原生药粉微生物检测的问题。(The invention provides a method for detecting microorganisms of primary medicinal powder, which belongs to the field of traditional Chinese medicine, and specifically comprises the steps of directly inoculating a test sample into a culture medium for culture; taking the culture solution to an RV salmonella enrichment liquid culture medium for secondary culture; the culture is inoculated to a xylose lysine deoxycholate agar culture medium to be cultured to obtain a suspected colony. The suspected bacterial colony is selected by the inoculating needle to be carried out slant and high-level puncture inoculation culture on the high-level slant of the trisaccharide iron agar culture medium, so as to judge the condition of the microorganism according to the result.)