阅读说明：本技术 一种小麦多肽的提取及制备方法 (Extraction and preparation method of wheat polypeptide ) 是由 李成秀 张宗方 于 2021-02-15 设计创作，主要内容包括：本发明公开了一种小麦多肽的提取及制备方法,本发明涉及小麦多肽提取及制备技术领域,Q1.原料选择：取新鲜小麦仁20粒,将小麦仁用清水清洗干净,然后用热风烘干,Q2.将烘干后的小麦仁放入液氮瓶中2s,再用镊子取出冷冻过的小麦仁；Q3.用研磨机将小麦仁粉碎,得到麦仁粉末,麦仁粉末注入玻璃杯中；Q4.向玻璃杯中加入清水,再用小型电动打蛋器快速搅拌清水和麦仁粉末；本发明中,利用液氮急速冷冻小麦仁,有利于短时间内得到小麦仁粉末,利用40目筛和200目筛对麦仁浆进行两次过滤,去除浆料内的杂质,在对麦仁浆进行蛋白水解的过程中,首先加入沙雷肽酶,在中性条件下进行初步酶解,提高了肽液纯度。(The invention discloses a method for extracting and preparing wheat polypeptide, which relates to the technical field of extraction and preparation of wheat polypeptide, wherein Q1. raw materials are selected as follows: taking 20 fresh wheat kernels, cleaning the wheat kernels with clear water, drying the wheat kernels with hot air, and placing the dried wheat kernels into a liquid nitrogen bottle for 2s, and taking out the frozen wheat kernels with tweezers; q3. grinding semen Tritici aestivi with grinder to obtain semen Tritici aestivi powder, and filling the semen Tritici aestivi powder into glass; q4, adding clear water into the glass cup, and rapidly stirring the clear water and the wheat kernel powder by using a small electric egg beater; according to the invention, wheat kernels are rapidly frozen by using liquid nitrogen, which is beneficial to obtaining wheat kernel powder in a short time, wheat kernel slurry is filtered twice by using a 40-mesh sieve and a 200-mesh sieve, impurities in the slurry are removed, and in the process of carrying out proteolysis on the wheat kernel slurry, Serratin peptidase is firstly added, and preliminary enzymolysis is carried out under a neutral condition, so that the purity of peptide liquid is improved.)

1. The extraction and preparation method of the wheat polypeptide is characterized by comprising the following steps:

q1. raw material selection: taking 20 fresh wheat kernels, cleaning the wheat kernels with clear water, and then drying the wheat kernels with hot air;

q2, putting the dried wheat kernels into a liquid nitrogen bottle for 2s, and taking out the frozen wheat kernels by using tweezers;

q3. grinding semen Tritici aestivi with grinder to obtain semen Tritici aestivi powder, and filling the semen Tritici aestivi powder into glass;

q4, adding clear water into the glass cup, and rapidly stirring the clear water and the wheat kernel powder by using a small electric egg beater;

q5. sieving the stirred semen Tritici aestivi powder solution with 40 mesh sieve to obtain semen Tritici aestivi powder crude solution, and sieving the semen Tritici aestivi powder crude solution with 200 mesh sieve to obtain semen Tritici aestivi powder refined solution;

q6, injecting the wheat kernel powder essence solution into a glass cup, and putting the glass cup into a refrigerator for cold storage for 5 min;

taking out the refrigerated wheat kernel powder extract solution, and adding 1g of serratipeptidase, 1g of alkaline protease and 0.5g of trypsin into a glass cup to decompose the protein in the wheat kernel powder extract solution to obtain an extracting solution after the protein is decomposed;

q8. injecting the extractive solution into an ultrafiltration membrane device, powering with a vacuum pump, and filtering with the ultrafiltration membrane device to obtain peptide solution;

q9. vacuum drying the peptide liquid to obtain the final product wheat polypeptide.

2. The method for extracting and preparing wheat polypeptide according to claim 1, wherein the method comprises the following steps: in the treatment of step Q4, 10ml of clean water was added.

3. The method for extracting and preparing wheat polypeptide according to claim 1, wherein the method comprises the following steps: the wheat kernel powder extract solution obtained after the treatment of the step Q5 was 4 ml.

4. The method for extracting and preparing wheat polypeptide according to claim 1, wherein the method comprises the following steps: the extract obtained after the treatment in step Q7 was 2.5 ml.

5. The method for extracting and preparing wheat polypeptide according to claim 1, wherein the method comprises the following steps: the amount of the peptide solution obtained after the treatment in step Q8 was 1.5 ml.

6. The method for extracting and preparing wheat polypeptide according to claim 1, wherein the method comprises the following steps: in the step Q9, the vacuum drying environment temperature is 0-5 ℃ and the air pressure is 23 MPa.

Technical Field

The invention belongs to the technical field of extraction and preparation of wheat polypeptides, and particularly relates to an extraction and preparation method of wheat polypeptides.

Background

Wheat is a cereal crop widely planted around the world, wheat caryopsis are ground into flour and then can be made into foods such as bread, steamed bread, biscuits, noodles and the like, fermentation can be carried out to prepare beer, alcohol and white spirit, the wheat contains a large amount of wheat oligopeptide, the wheat oligopeptide is wheat protein and is obtained through protease enzymolysis, the wheat oligopeptide is obtained through crushing wheat kernels and then baking at high temperature, mixing and filtering in the existing wheat oligopeptide extraction process, the activity of the protein can be damaged in the baking process, the yield of the protein is reduced, the final polypeptide yield is reduced, and after mixing, the wheat kernel slurry is directly decomposed, a large amount of protease is needed to decompose the protein, and the control of the using amount of the protease is not facilitated.

Disclosure of Invention

Accordingly, the present invention is directed to a method for extracting and preparing wheat polypeptide, which effectively solves the problems of the above background art that the activity of protein is destroyed during baking, the yield of protein is reduced, and after size mixing, the wheat kernel pulp is directly decomposed, and the use of a large amount of protease is disadvantageous for controlling the use amount of protease.

In order to achieve the purpose, the invention provides the following technical scheme: a method for extracting and preparing wheat polypeptide comprises the following steps:

q1. raw material selection: taking 20 fresh wheat kernels, cleaning the wheat kernels with clear water, and then drying the wheat kernels with hot air;

q2, putting the dried wheat kernels into a liquid nitrogen bottle for 2s, and taking out the frozen wheat kernels by using tweezers;

q3. grinding semen Tritici aestivi with grinder to obtain semen Tritici aestivi powder, and filling the semen Tritici aestivi powder into glass;

q4, adding clear water into the glass cup, and rapidly stirring the clear water and the wheat kernel powder by using a small electric egg beater;

q5. sieving the stirred semen Tritici aestivi powder solution with 40 mesh sieve to obtain semen Tritici aestivi powder crude solution, and sieving the semen Tritici aestivi powder crude solution with 200 mesh sieve to obtain semen Tritici aestivi powder refined solution;

q6, injecting the wheat kernel powder essence solution into a glass cup, and putting the glass cup into a refrigerator for cold storage for 5 min;

taking out the refrigerated wheat kernel powder extract solution, and adding 1g of serratipeptidase, 1g of alkaline protease and 0.5g of trypsin into a glass cup to decompose the protein in the wheat kernel powder extract solution to obtain an extracting solution after the protein is decomposed;

q8. injecting the extractive solution into an ultrafiltration membrane device, powering with a vacuum pump, and filtering with the ultrafiltration membrane device to obtain peptide solution;

q9. vacuum drying the peptide liquid to obtain the final product wheat polypeptide.

Preferably, in the treatment of step Q4, 10ml of clean water is added.

Preferably, the wheat kernel powder extract solution obtained after the treatment of the step Q5 is 4 ml.

Preferably, the amount of the extract obtained after the treatment in step Q7 is 2.5 ml.

Preferably, the amount of the peptide solution obtained after the treatment in step Q8 is 1.5 ml.

Preferably, in the step Q9 treatment, the vacuum drying environment temperature is 0-5 ℃ and the air pressure is 23 MPa.

Compared with the prior art, the invention has the beneficial effects that:

(1) according to the invention, the wheat kernels are rapidly frozen by using liquid nitrogen, and then are rapidly ground by using the grinder, so that the wheat kernel powder can be obtained in a short time, the speed of extracting the wheat kernels is improved, the period is shortened, the operation is simpler, and the protein in the wheat kernels is protected.

(2) In the invention, the wheat kernel pulp is filtered twice by using a 40-mesh sieve and a 200-mesh sieve, so that impurities in the pulp are removed, the using amount of the protease is reduced, the full contact mixing reaction of protein and the protease is facilitated, the using amount of the protease is convenient to control, and the operation difficulty of the protein hydrolysis treatment is reduced.

(3) In the invention, in the process of carrying out proteolysis on wheat kernel slurry, firstly adding serrapeptase, carrying out preliminary enzymolysis under neutral conditions, then adding alkaline protease for enzymolysis of protein and decomposing lipid substances in the wheat kernel slurry, and finally adding trypsin for enzymolysis of residual protein, thereby improving the enzymolysis precision, reducing impurities in an extracting solution after enzymolysis and improving the purity of peptide liquid.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.

The invention provides the following technical scheme:

the first embodiment is as follows:

1. a method for extracting and preparing wheat polypeptide comprises the following steps:

q1. raw material selection: taking 20 fresh wheat kernels, cleaning the wheat kernels with clear water, and then drying the wheat kernels with hot air;

q2, putting the dried wheat kernels into a liquid nitrogen bottle for 2s, and taking out the frozen wheat kernels by using tweezers;

q3. grinding semen Tritici aestivi with grinder to obtain semen Tritici aestivi powder, and filling the semen Tritici aestivi powder into glass;

q4, adding clear water into the glass cup, and rapidly stirring the clear water and the wheat kernel powder by using a small electric egg beater;

q5. sieving the stirred semen Tritici aestivi powder solution with 40 mesh sieve to obtain semen Tritici aestivi powder crude solution, and sieving the semen Tritici aestivi powder crude solution with 200 mesh sieve to obtain semen Tritici aestivi powder refined solution;

q6, injecting the wheat kernel powder essence solution into a glass cup, and putting the glass cup into a refrigerator for cold storage for 5 min;

taking out the refrigerated wheat kernel powder extract solution, and adding 1g of serratipeptidase, 1g of alkaline protease and 0.5g of trypsin into a glass cup to decompose the protein in the wheat kernel powder extract solution to obtain an extracting solution after the protein is decomposed;

q8. injecting the extractive solution into an ultrafiltration membrane device, powering with a vacuum pump, and filtering with the ultrafiltration membrane device to obtain peptide solution;

q9. vacuum drying the peptide liquid to obtain the final product wheat polypeptide.

Example two:

a method for extracting and preparing wheat polypeptide comprises the following steps:

q1. raw material selection: taking 20 fresh wheat kernels, cleaning the wheat kernels with clear water, and then drying the wheat kernels with hot air;

q2, placing the dried wheat kernels into a microwave oven for 10min to remove excessive water;

q3. grinding semen Tritici aestivi with grinder to obtain semen Tritici aestivi powder, and filling the semen Tritici aestivi powder into glass;

q4, adding clear water into the glass cup, and rapidly stirring the clear water and the wheat kernel powder by using a small electric egg beater;

q5. sieving the stirred semen Tritici aestivi powder solution with 40 mesh sieve to obtain semen Tritici aestivi powder crude solution;

q6, injecting the wheat kernel powder essence solution into a glass cup, and putting the glass cup into a refrigerator for cold storage for 5 min;

taking out the refrigerated wheat kernel powder extract solution, and then adding serrapeptase into the glass cup to decompose the protein in the wheat kernel powder extract solution to obtain an extracting solution after the protein is decomposed;

q8. injecting the extractive solution into an ultrafiltration membrane device, powering with a vacuum pump, and filtering with the ultrafiltration membrane device to obtain peptide solution;

q9. vacuum drying the peptide liquid to obtain the final product wheat polypeptide.

For the 2 embodiments described above:

comparing the times of obtaining the wheat kernel powder after freezing and heating, the following table is obtained:

as can be seen from the above table, the method provided by the present invention can significantly increase the speed of obtaining wheat kernel powder and improve the speed of extracting wheat kernels.

Comparing the protease dosage in the enzymolysis of the wheat kernel pulp subjected to the primary filtration and the secondary filtration, the following table is obtained:

as can be seen from the above table, the amount of protease consumed by the treatment of the wheat kernel slurry by the method provided by the invention is obviously reduced, the use level of the protease is convenient to control by a user, the operation is simpler, the impurities in the extracting solution after enzymolysis are reduced, and the purity of the peptide liquid is improved.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.