阅读说明：本技术 有序金立方纳米簇以及有序金立方纳米簇的制备方法 (Ordered gold cubic nanocluster and preparation method thereof ) 是由 晁洁 高霏 汪联辉 于 2021-04-08 设计创作，主要内容包括：本发明公开了一种有序金立方纳米簇以及有序金立方纳米簇的制备方法,属于DNA纳米技术领域。有序金立方纳米簇包括DNA折纸单体与金立方单聚体,DNA折纸单体上设有捕获链和连接边链,捕获链用于连接金立方单聚体；连接边链包括特定序列的黏性末端,用于以互补连接的方式将多个DNA折纸单体组装成DNA折纸框架。本发明的DNA折纸单体通过特定序列的黏性末端互补实现连接,组装成预设形状的DNA折纸框架,解决了DNA折纸技术构建平台时的尺寸缺陷,进一步的,通过在DNA折纸单体上连接金立方单聚体,形成预设形状的有序金立方纳米簇；同时,DNA折纸框架还具备手形,辅助杂交其他纳米粒子得到具有耦合效应的聚集体,可能得到在宏观尺度内无法得到的特殊光学现象。(The invention discloses an ordered gold cubic nano-cluster and a preparation method thereof, belonging to the technical field of DNA nanometer. The ordered gold cubic nanoclusters comprise DNA origami monomers and gold cubic monomers, wherein capture chains and connecting side chains are arranged on the DNA origami monomers, and the capture chains are used for connecting the gold cubic monomers; the connecting side chain comprises adhesive ends with specific sequences, and is used for assembling a plurality of DNA origami monomers into a DNA origami frame in a complementary connection mode. The DNA paper folding monomers are connected through the complementary realization of the cohesive ends of the specific sequences to assemble the DNA paper folding frame with the preset shape, thereby solving the size defect when the DNA paper folding technology constructs a platform, and further forming the ordered gold cubic nanoclusters with the preset shape by connecting the gold cubic monomers on the DNA paper folding monomers; meanwhile, the DNA paper folding frame is also in a hand shape, and assists in hybridizing other nano particles to obtain an aggregate with a coupling effect, so that a special optical phenomenon which cannot be obtained in a macroscopic scale can be obtained.)

1. An ordered cubic gold nanocluster characterized by: the ordered gold cubic nanoclusters comprise DNA origami monomers and gold cubic monomers, wherein capture chains and connecting side chains are arranged on the DNA origami monomers, and the capture chains are used for connecting the gold cubic monomers; the connecting side chain comprises adhesive ends with specific sequences, and is used for assembling a plurality of DNA origami monomers into a DNA origami frame in a complementary connection mode.

2. The ordered cubic gold nanocluster of claim 1, wherein: DNA paper folding monomer is cross DNA paper folding monomer, the setting of connecting side chain is in the free edge of cross DNA paper folding, just the free at least one end of cross DNA paper folding is provided with connecting side chain.

3. The ordered cubic gold nanocluster of claim 1, wherein: the DNA origami double-stranded paper is characterized in that the number of the connecting side chains is six, the six connecting side chains are complementary through the cohesive ends of specific sequences to form three sets of connecting double chains, and at least one set of connecting double chains is formed among a plurality of DNA origami monomers.

4. The ordered cubic gold nanocluster of claim 1, wherein: the DNA paper folding monomer comprises an A-type DNA paper folding monomer and a B-type DNA paper folding monomer, and the A-type DNA paper folding monomer rotates by 90 degrees to obtain the B-type DNA paper folding monomer.

5. The ordered cubic gold nanocluster of claim 4, wherein: the sticky ends of the left and right specific sequences of the A-type DNA paper folding monomer are complemented with the sticky ends of the left and right specific sequences of the B-type DNA paper folding monomer to realize connection, and the sticky ends of the upper and lower specific sequences of the A-type DNA paper folding monomer are complemented with the sticky ends of the upper and lower specific sequences of the B-type DNA paper folding monomer to realize connection.

6. A preparation method of ordered gold cubic nanoclusters, which is applied to the ordered gold cubic nanoclusters of any one of claims 1 to 5, and is characterized by comprising the following steps:

s1, preparing DNA paper folding monomers, wherein the DNA paper folding monomers comprise A type DNA paper folding monomers and B type DNA paper folding monomers;

s2, mixing the A-type DNA paper folding monomer and the B-type DNA paper folding monomer, and annealing to obtain a DNA paper folding frame;

s3, preparing a golden cubic monomer;

s4, assembling the golden cubic monomers with the A-type DNA origami monomers and the B-type DNA origami monomers respectively to obtain A-type DNA origami-golden cubic monomers and B-type DNA origami-golden cubic monomers respectively;

S5, mixing the A-type DNA origami-golden cubic monomer and the B-type DNA origami-golden cubic monomer, and annealing to obtain the ordered golden cubic nano-cluster.

7. The method for preparing the ordered gold cubic nanoclusters of claim 6, wherein the step S1 is specifically: uniformly mixing the skeleton chain, the staple chain and the modified chain in proportion, placing the mixture in a polymerase chain reaction instrument, annealing the mixture from 95 ℃ to 20 ℃ at the speed of 0.1 ℃/10s, and performing ultrafiltration and purification treatment to obtain the DNA origami monomer.

8. The method for preparing the ordered gold cubic nanoclusters of claim 6, wherein the step S2 is specifically: and uniformly mixing the A-type DNA origami monomer and the B-type DNA origami monomer in proportion, and annealing in a water bath at 55 ℃ to room temperature to obtain the DNA origami frame.

9. The method for preparing the ordered gold cubic nanoclusters of claim 6, wherein the step S4 is specifically: and (3) assembling the golden cubic monomers with the A-type DNA origami monomers and the B-type DNA origami monomers respectively, repeatedly annealing for four times from 45 ℃ to 30 ℃ until the temperature is reduced to 4 ℃, and purifying to obtain the A-type DNA origami-golden cubic monomers and the B-type DNA origami-golden cubic monomers respectively.

10. The method for preparing the ordered gold cubic nanoclusters of claim 6, wherein the step S5 is specifically: and after the A-type DNA origami-cube unimer and the B-type DNA origami-cube unimer are uniformly mixed, naturally annealing from 55 ℃ to 25 ℃ to obtain the ordered gold cube nano-cluster.

Technical Field

The invention relates to an ordered gold cubic nano-cluster and a preparation method thereof, belonging to the technical field of DNA nano-technology.

Background

At present, self-assembly of nano-scale objects into a preset shape structure from bottom to top is a very important direction in the nano field, which means that human beings can precisely regulate and control nano-scale particles on a microscopic level by using a concept of crossing scales, and a plurality of ordered nano materials constructed by the method are superior to traditional materials in terms of optical properties, and an industrial innovation is possibly brought.

DNA nanotechnology is an emerging interdisciplinary discipline in recent years. In 1982, professor naddrian Seeman innovatively proposed a concept: the existence of branched nanostructures that can self-assemble by exploiting the specific base complementary pairing ability of DNA has marked the advent of DNA nanotechnology. Rothmund proposed a new self-assembly strategy in 2006, whose basic idea was to fold a long DNA single strand (M13mp18) into a specific shape and to fix the shape by hybridization with many short "staple strands", which is called DNA origami (DNA origami) technology, which is a milestone of DNA nanotechnology and attracts extensive attention from scientists in different research fields such as chemistry, biology, and materials science. In the short few years, the research based on DNA origami has been remarkably developed, and bewildering nanopatterns and nanostructures are produced, so that the advantages of the DNA origami self-assembly technology are fully demonstrated.

DNA nanotechnology aims at organizing substances with the highest accuracy and control that will lead to nanoelectronics, nanotechnology, programmable chemical synthesis, scaffolding crystals, and nanoscopic systems that respond to the environment. Structured DNA nanotechnology is one of the most powerful ways to achieve this goal, combining powerful branched DNAs with programmable cohesive ends and affinity control, whose success includes the formation of substances: 2D crystals, 3D crystals, nanomechanical devices, and various combinations of these. However, for complex nanostructures, conventional nanoscale fabrication methods have low repeatability, are time consuming, are complex, and cannot really achieve precise control on the nanoscale, and some structurally and functionally diverse nanomolecular devices and machines are limited and affected by the size of the building platform.

In view of the above, it is necessary to provide a super-assembly method based on DNA origami technology to solve the above problems.

Disclosure of Invention

The invention aims to provide an ordered gold cubic nano-cluster and a preparation method of the ordered gold cubic nano-cluster, and the method can super-assemble a molecular assembly body in any shape based on a DNA paper folding technology.

In order to achieve the purpose, the invention provides an ordered gold cubic nano-cluster, which comprises a DNA origami monomer and a gold cubic monomer, wherein the DNA origami monomer is provided with a capturing chain and a connecting side chain, and the capturing chain is used for connecting the gold cubic monomer; the connecting side chain comprises adhesive ends with specific sequences, and is used for assembling a plurality of DNA origami monomers into a DNA origami frame in a complementary connection mode.

As a further improvement of the invention, the DNA origami monomer is a cross-shaped DNA origami monomer, the connecting side chain is arranged at the edge of the cross-shaped DNA origami monomer, and at least one end of the cross-shaped DNA origami monomer is provided with the connecting side chain.

As a further improvement of the invention, the connecting side chains are provided with six types, the six types of connecting side chains are complemented through cohesive ends of specific sequences to form three sets of connecting double chains, and at least one set of connecting double chains is formed among a plurality of DNA origami monomers.

As a further improvement of the invention, the DNA origami monomer comprises an A-type DNA origami monomer and a B-type DNA origami monomer, and the A-type DNA origami monomer is rotated by 90 degrees to obtain the B-type DNA origami monomer.

As a further improvement of the invention, the cohesive ends of the left and right specific sequences of the A-type DNA origami monomer are complementarily connected with the cohesive ends of the left and right specific sequences of the B-type DNA origami monomer, and the cohesive ends of the upper and lower specific sequences of the A-type DNA origami monomer are complementarily connected with the cohesive ends of the upper and lower specific sequences of the B-type DNA origami monomer.

In order to realize the purpose, the invention also provides a preparation method of the ordered cubic gold nanocluster, which comprises the following steps:

s1, preparing DNA paper folding monomers, wherein the DNA paper folding monomers comprise A type DNA paper folding monomers and B type DNA paper folding monomers;

s2, mixing the A-type DNA paper folding monomer and the B-type DNA paper folding monomer, and annealing to obtain a DNA paper folding frame;

s3, preparing a golden cubic monomer;

s4, assembling the golden cubic monomers with the A-type DNA origami monomers and the B-type DNA origami monomers respectively to obtain A-type DNA origami-golden cubic monomers and B-type DNA origami-golden cubic monomers respectively;

s5, mixing the A-type DNA origami-golden cubic monomer and the B-type DNA origami-golden cubic monomer, and annealing to obtain the ordered golden cubic nano-cluster.

As a further improvement of the present invention, step S1 specifically includes: uniformly mixing the skeleton chain, the staple chain and the modified chain in proportion, placing the mixture in a polymerase chain reaction instrument, annealing the mixture from 95 ℃ to 20 ℃ at the speed of 0.1 ℃/10s, and performing ultrafiltration and purification treatment to obtain the DNA origami monomer.

As a further improvement of the present invention, step S2 specifically includes: and uniformly mixing the A-type DNA origami monomer and the B-type DNA origami monomer in proportion, and annealing in a water bath at 55 ℃ to room temperature to obtain the DNA origami frame.

As a further improvement of the present invention, step S4 specifically includes: and (3) assembling the golden cubic monomers with the A-type DNA origami monomers and the B-type DNA origami monomers respectively, repeatedly annealing for four times from 45 ℃ to 30 ℃ until the temperature is reduced to 4 ℃, and purifying to obtain the A-type DNA origami-golden cubic monomers and the B-type DNA origami-golden cubic monomers respectively.

As a further improvement of the present invention, step S5 specifically includes: and after the A-type DNA origami-cube unimer and the B-type DNA origami-cube unimer are uniformly mixed, naturally annealing from 55 ℃ to 25 ℃ to obtain the ordered gold cube nano-cluster.

The invention has the beneficial effects that: the DNA paper folding monomers are connected through the complementary realization of the cohesive ends of the specific sequences to assemble the DNA paper folding frame with the preset shape, thereby solving the size defect when the DNA paper folding technology constructs a platform, and further forming the ordered gold cubic nanoclusters with the preset shape by connecting the gold cubic monomers on the DNA paper folding monomers; meanwhile, the DNA paper folding frame is also in a hand shape, and assists in hybridizing other nano particles to obtain an aggregate with a coupling effect, so that a special optical phenomenon which cannot be obtained in a macroscopic scale can be obtained.

Drawings

FIG. 1 is a schematic diagram of the formation of anisotropic A-type DNA origami monomers and B-type DNA origami monomers in the present invention.

FIG. 2 is a design diagram of DNA origami frame assembled by type A DNA origami monomers and type B DNA origami monomers in FIG. 1.

FIG. 3 is an atomic force microscope representation of the DNA origami framework of FIG. 2.

FIG. 4 is a schematic diagram of the assembly of a DNA origami-gold cubic monomer with a DNA origami monomer according to the present invention.

FIG. 5 is an agarose gel electrophoresis of the DNA origami type A-gold cubic monomers and the DNA origami type B-gold cubic monomers of FIG. 4.

FIG. 6 is a schematic diagram of the mixture of type A DNA origami-auricle monomers and type B DNA origami-auricle monomers in FIG. 4 to form TE-T ordered gold cubic nanoclusters.

FIG. 7 is a transmission electron microscopy characterization of the TE-T ordered gold cubic nanoclusters of FIG. 6.

FIG. 8 is a transmission electron microscopy characterization of ordered gold cubic nanoclusters of the present invention.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in detail with reference to the accompanying drawings and specific embodiments.

The invention provides an ordered gold cubic nano-cluster and a preparation method thereof, which mainly comprises the following steps:

S1: preparing DNA origami monomers with anisotropy and different types.

Referring to fig. 1, annealing the skeleton chain, the staple chain, and the modified chain to form a DNA origami monomer, wherein the DNA origami monomer is a cross-shaped DNA origami monomer, and the DNA origami monomer includes an a-type DNA origami monomer and a B-type DNA origami monomer, and the a-type DNA origami monomer rotates 90 ° to obtain a B-type DNA origami monomer; the skeleton chain is single-stranded DNA of bacteriophage M13mp 18; the staple chain is 176 rack chains with the side chain removed and the chain modified; the modified chains comprise 8 capturing chains and a plurality of connecting side chains, wherein the 8 capturing chains are scaffold chains of 5' modified polyA and are used for capturing the golden cubic monomers; the connecting side chain comprises a sticky end with a specific sequence, and at least one end of the cross-shaped DNA paper folding monomer is provided with the connecting side chain so as to form different types of DNA paper folding monomers.

In this embodiment, there are six types of connecting side chains, which are defined as: 1. 1', 2', 3 and 3', wherein the six connecting side chains are complementarily connected through the cohesive ends of the specific sequences to form three sets of connecting double chains, specifically, 1 and 1' can be complemented to form one set of connecting double chains, 2 and 2 'can be complemented to form one set of connecting double chains, and 3' can be complemented to form one set of connecting double chains, so as to realize the anisotropy of the DNA origami monomers.

In this embodiment, the sticky ends of the left and right specific sequences of the a-type DNA origami monomer are complementary with the sticky ends of the left and right specific sequences of the B-type DNA origami monomer to realize connection, the sticky ends of the upper and lower specific sequences of the a-type DNA origami monomer are complementary with the sticky ends of the upper and lower specific sequences of the B-type DNA origami monomer to realize connection, specifically, the connecting side chain on the a-type DNA origami monomer is one or more of 1, 2 and 3, and the connecting side chain on the B-type DNA origami monomer is one or more of 1', 2' and 3 '.

In this embodiment, the connection side chain includes six specific sequences's stickness end to realize stable the connection between a plurality of DNA paper folding monomers, certainly, in other embodiments, can set up many specific sequences's stickness end on the connection side chain, as long as can realize the stable connection between a plurality of DNA paper folding monomers.

The method comprises the following specific steps:

s11: the skeleton chain, staple chain and modified chain were mixed at a molar ratio of 1:10:10, i.e., added in volumes of 2.5. mu.L, 5. mu.L, respectively, followed by 10. mu.L of 10 XTAE/Mg²⁺Buffer solution (Mg)²⁺Concentration of 12.5mol/L), supplementing ultrapure water to a final volume of 100 mu L, and shaking up.

S12: the mixed solution in S11 was placed in a PCR machine (polymerase chain reaction) to anneal from 95 ℃ to 20 ℃ at a rate of 0.1 ℃/10 sec.

S13: the annealed product is purified to remove excess short DNA strands.

S14: the sample obtained in S13 was subjected to ultrafiltration purification in a 100kDa ultrafiltration tube and supplemented with 1 XTAE/Mg²⁺(the mixture ratio is 40mM trihydroxymethyl aminomethane, 20mM acetic acid, 2mM disodium ethylenediamine tetraacetic acid and 12.5mM magnesium acetate) to 400 μ L. Centrifuging at 3000g for 10 min, discarding the filtrate after each centrifugation, and supplementing with 1 XTAE/Mg²⁺After the buffer solution is adjusted to 400 μ L and the centrifugation is repeated three times, the ultrafiltration tube is reversely buckled in a centrifugal tube of 1.5mL, and is centrifuged for 10 minutes under the condition of 1000g, and the filtrate, i.e. A, B type DNA origami monomers, is collected, and is respectively: a. the_R3、A_R1、A_U2R1、A_R1L1、A_D2、B_L3'D2'、B_L1'、B_U2'L1'、B_R1'U2'L1'、B_D2'… …, and standing the filtrate at 4 deg.C.

In this example, only ten types of A, B-type DNA origami monomers are exemplified, but this should not be construed as a limitation as long as the DNA origami monomers are anisotropic and different in kind. For example, A may also be included_R1L3、A_R3D2、A_U2L3、A_U2R3、B_L1'R3'、B_L3'And other types of DNA origami monomers.

S15: quantifying each DNA origami monomer by using an ultramicro-spectrophotometer to obtain an absorbance value A₂₆₀。

S2: mixing the A-type DNA origami monomer and the B-type DNA origami monomer, and annealing to prepare different types of DNA origami frames for preliminarily verifying the rationality of the preparation method.

Referring to fig. 2, the DNA origami monomers are mixed according to the design ratio, and complementary pairing is performed on the viscous ends of the specific sequence in the solution, in this embodiment, only seven DNA origami frames with preset shapes are listed, which are respectively: TE-I, TE-T, TE-O, TE-Z, TE-S, TE-L and TE-J, however, in other embodiments, DNA origami frames of other preset shapes may be formed, and the shape of the DNA origami frame is not limited.

Wherein A is_R1、B_L1'R3'、A_R1L3And B_L1'Mixing the materials according to the proportion of 1:1:1:1, and annealing to obtain the TE-I type DNA origami framework.

A_R1、B_R1'U2'L1'And A_D2Mixing the materials according to the proportion of 2:1:1, and annealing to obtain the TE-T type DNA origami frame.

A_U2R1And B_U2'L1'And mixing according to the proportion of 2:2, and annealing to obtain the TE-O type DNA origami frame.

A_R3、B_L3'D2'、A_U2R1And B_L1'Mixing the materials according to the proportion of 1:1:1:1, and annealing to obtain the TE-Z type DNA origami framework.

A_R3D2、B_L3'、A_R1And B_U2'L1'Mixing the materials according to the proportion of 1:1:1:1, and annealing to obtain the TE-S type DNA origami framework.

A_U2L3、B_D2'、A_U2R3And B_D2'The TE-L type DNA origami framework and the TE-J type DNA origami framework can be obtained by mixing and annealing according to the proportion of 1:1:1:1, and the TE-L type DNA origami framework and the TE-J type DNA origami framework are mutually hand-shaped frameworks.

The method comprises the following specific steps:

s21: according to the shape structure of each DNA origami frame, at 1 XTAE/Mg²⁺In the buffer solution, the corresponding DNA origami monomers are mixed according to the molar ratio.

S22: and (3) putting the mixed solution in the S21 into a water bath at 55 ℃ for incubation, and naturally cooling the water to room temperature for about 13 hours to form the DNA origami frame in the preset shape.

As shown in FIG. 3, 5. mu.L of the sample obtained in S22 was dropped on a flat freshly peeled mica sheet, adsorbed for 2min, rinsed with ultrapure water, dried with an ear-washing ball gently in one direction, and then observed under a gas phase condition of an atomic force microscope to verify the DNA origami frame of the predetermined shape obtained in S22, and FIG. 3 included seven shapes of the DNA origami frames in this example.

The model of the atomic force microscope and the parameters set during scanning are as follows: multimodal type 8 atomic force microscope (NanoScope type 5 controller, Bruker corporation); scanning with TESPA-V2 probe in tapping mode at a scanning range of 3 μm and a scanning frequency of 1H_ZThe scan resolution is 384.

S3, preparing a golden cubic monomer.

After the gold cubic nanoparticles with the specification of 50nm are centrifugally concentrated, adding a sulfhydryl DNA single chain in a buffer solution environment, and finishing the assembly of the sulfhydryl DNA single chain and the gold cubic nanoparticles in an aging mode of adding salt (NaCl) to obtain the gold cubic nanoparticles with the surface covered with the sulfhydryl DNA single chain, namely the gold cubic monomers.

The method comprises the following specific steps:

s31: 800. mu.L of 50nm Kindothel nanoparticles were put into a 1.5mL centrifuge tube, centrifuged at 5000rpm for 10min, the supernatant was removed, 370. mu.L of ultrapure water, 5. mu.L of SDS (1%) stabilizer and 10. mu.L of thiol DNA single strand were added to the centrifuge tube, and the mixture was shaken and mixed.

S32: the sample in S31 was placed in a homogenizer and incubated at 37 ℃ for 6 hours at 300 rpm.

S33: to the incubated sample of S32, 50. mu.L of 5 XTBE (89mM Tris, 89mM acetic acid, 2mM disodium EDTA, pH 8.0) buffer solution was added, and mixed by shaking.

S34: after 30 minutes, 50. mu.L of 3M NaCl solution was added to the mixed solution of S33 in four times, and 3M NaCl solution was added in volumes of 5, 10, 15 and 20. mu.L, respectively, at intervals of 30 minutes, and incubated at 37 ℃ overnight.

Adding NaCl solution into the mixed solution step by step, on one hand, in order to ensure that the sulfhydryl DNA single chain can be assembled on the surface of the gold cubic nanoparticle; on the other hand, for the purpose of aging, the adsorption between the surface of the gold cubic nanoparticle and the thiol DNA single strand is reduced, so that the thiol DNA single strand tends to stand on the surface of the gold cubic nanoparticle, thereby increasing the assembly amount of the thiol DNA single strand.

S35: the sample from S34 was centrifuged at 5000rpm for 10min, the centrifugation was repeated four times, the supernatant was discarded after each centrifugation, and 0.5 XTBE (45mM Tris, 45mM acetic acid, 1mM disodium EDTA, pH 8.5) was added to 500. mu.L. And (4) centrifuging for the fourth time, and then removing the supernatant to obtain the golden cubic monomer.

S4, assembling the golden cubic monomers with the A-type DNA origami monomers and the B-type DNA origami monomers respectively to obtain the A-type DNA origami-golden cubic monomers respectively.

Referring to fig. 4, the specific steps include:

s41: mixing the A-type and B-type DNA origami monomers obtained in S15 with excessive amount of the golden cubic monomer obtained in S35, annealing at 45-30 deg.C for 4 times, and cooling to 4 deg.C.

S42: referring to fig. 5, 0.8% agarose gel is prepared, the product obtained in S41 is electrophoresed at 90v for 90 minutes, the target band of the assembled product is cut off after electrophoresis for 90 minutes, the cut-off target band is put into a dialysis bag, electrophoresis is continued at 90v for 45 minutes, and the solution in the dialysis bag is recovered, i.e., the a-type and B-type DNA origami-gold cubic monomers.

S5, mixing the A-type and B-type DNA origami-cube monomers, and annealing to obtain the ordered gold cube nano-cluster.

Taking TE-T type ordered gold cubic nanoclusters as an example, please refer to FIGS. 6 and 7, and the A type and B type DNA origami-gold cubic monomers obtained in S42 are processed according to A_R1：B_R1'U2'L1'：A_D2And (3) annealing after mixing in a ratio of 2:1:1 to obtain the TE-T type ordered gold cubic nano-cluster, and observing by using a transmission electron microscope to obtain a transmission electron microscope characterization result of the TE-T type ordered gold cubic nano-cluster.

The method comprises the following specific steps:

s51: mixing the A-type and B-type DNA origami-gold cubic monomers obtained in the step S42 according to the design proportion in the step S2, and naturally annealing the mixture in a water bath at 55 ℃ to room temperature.

S52: the product obtained in S51 was observed under a transmission electron microscope. The method specifically comprises the following steps: and (3) dropping 10 mu L of sample on the surface of the carbon supporting film to form liquid beads, adsorbing for 15 minutes, sucking the sample away by using a liquid transfer gun, washing the carbon supporting film twice by using ultrapure water, placing the carbon supporting film to room temperature, drying the sample, and performing characterization imaging by using a transmission electron microscope to obtain the TE-T type ordered gold cubic nanoclusters.

As shown in FIG. 8, the formation process of other six ordered cubic gold clusters is consistent with that of the TE-T type ordered cubic gold cluster, and finally, seven shapes of ordered cubic gold clusters of TE-I, TE-T, TE-O, TE-Z, TE-S, TE-LH and TE-J are obtained.

Although the present invention has been described in detail with reference to the preferred embodiments, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof without departing from the spirit and scope of the present invention.