阅读说明：本技术 注射用交联透明质酸钠凝胶无菌检测方法 (Sterility detection method for crosslinked sodium hyaluronate gel for injection ) 是由 王师亮 于 2020-12-07 设计创作，主要内容包括：本发明提供一种注射用交联透明质酸钠凝胶无菌检测方法,包括以下步骤：A1：制备菌悬液或孢子悬液；A2：供试品溶液的制备：先取含300IU/ml的玻璃酸酶溶液90ml,取10ml注射用交联透明质酸钠凝胶置玻璃酸酶溶液中,常温水解,水解过程中不断振摇,待凝胶完全水解后摇匀,即得。采用本发明提供的注射用交联透明质酸钠凝胶无菌检测方法,采用玻璃酸酶对供试品(样品)进行溶解,避免假阴性的问题,直接接种,由于凝胶不能溶解,可导致微生物不能很好的被检出,本无菌检测方法的准确率高,容易实现,适用于大规律推广。(The invention provides a method for aseptically detecting cross-linked sodium hyaluronate gel for injection, which comprises the following steps: a1: preparing a bacterial suspension or a spore suspension; a2: preparation of a test solution: taking 90ml of hyaluronidase solution containing 300IU/ml, taking 10ml of crosslinked sodium hyaluronate gel for injection, putting the crosslinked sodium hyaluronate gel into the hyaluronidase solution, hydrolyzing at normal temperature, continuously shaking in the hydrolysis process, and shaking up after the gel is completely hydrolyzed to obtain the hyaluronic acid gel. By adopting the sterile detection method of the cross-linked sodium hyaluronate gel for injection, provided by the invention, hyaluronidase is adopted to dissolve a sample to be tested, so that the problem of false negative is avoided, direct inoculation is carried out, and microorganisms cannot be well detected due to the fact that the gel cannot be dissolved.)

1. The method for detecting the sterility of the crosslinked sodium hyaluronate gel for injection is characterized by comprising the following steps:

a1: preparing a bacterial suspension or a spore suspension;

a2: preparation of a test solution: taking 90ml of hyaluronidase solution containing 300IU/ml, taking 10ml of crosslinked sodium hyaluronate gel for injection, putting the crosslinked sodium hyaluronate gel into the hyaluronidase solution, hydrolyzing at normal temperature, continuously shaking in the hydrolysis process, and shaking uniformly after the gel is completely hydrolyzed to obtain the hyaluronic acid gel;

a3: the detection method comprises the following steps:

a 1: preparation of test article groups: filtering 200ml of sample solution by using a dual disposable totally-enclosed bacteria collection culture device, adding 100ml of thioglycollate fluid medium (FTM) culture medium into one bacteria collection cup of the bacteria collection culture device after the completion, and adding 100ml of trypticase soy peptone liquid medium (TSB) culture medium into the other bacteria collection cup;

a 2: preparation of test article test group: performing the same operation with the test sample group, preparing 3 bacteria collection cups for each culture medium of a thioglycollate fluid medium (FTM) and a trypticase soy peptone liquid medium (TSB), respectively, totally 6 bacteria collection cups, transferring to a positive control detection room after completion, and respectively adding test bacteria less than l00 cfu; wherein, bacterial suspension or spore suspension of staphylococcus aureus, escherichia coli and clostridium sporogenes is added into a thioglycollate fluid medium (FTM) culture medium, and bacterial suspension or spore suspension of candida albicans, aspergillus niger and bacillus subtilis is added into a trypticase soy peptone liquid medium (TSB) culture medium;

a 3: preparation of positive control group: taking 3 cups of disposable sterile bacteria collection cups containing 100ml of thioglycollate fluid medium (FTM) and 100ml of trypticase soy peptone liquid medium (TSB) medium respectively, and adding test bacteria with the same amount as the bacteria added in the test group of the test sample to a positive control detection room; wherein, bacterial suspension or spore suspension of staphylococcus aureus, escherichia coli and clostridium sporogenes is added into a thioglycollate fluid medium (FTM) culture medium, and bacterial suspension or spore suspension of candida albicans, aspergillus niger and bacillus subtilis is added into a trypticase soy peptone liquid medium (TSB) culture medium;

a 4: preparation of negative control group: filtering 200ml of hyaluronidase solution containing 300IU/ml by using a dual disposable totally-enclosed bacteria collection culture device, adding 100ml of thioglycollate fluid medium (FTM) culture medium into one bacteria collection cup of the bacteria collection culture device after the filtration is finished, and adding 100ml of trypticase soy peptone liquid medium (TSB) culture medium into the other bacteria collection cup;

a 5: colony count confirmation group: test bacteria with the same amount as that added in the test group added with the test sample are taken and added into corresponding culture media, staphylococcus aureus, bacillus subtilis and escherichia coli are subjected to colony number confirmation by using a trypticase soytone agar medium (TSA), candida albicans and aspergillus niger are subjected to colony number confirmation by using a Sabouraud glucose agar medium (SDA), and clostridium sporogenes are subjected to colony number confirmation by using a thioglycollate fluid medium (FTM).

a 6: and (5) culturing.

2. The method for aseptically detecting cross-linked sodium hyaluronate gel for injection according to claim 1, wherein in step A1, Staphylococcus aureus, Escherichia coli, Clostridium sporogenes, Bacillus subtilis, Candida albicans and Aspergillus niger are made into bacterial suspension or spore suspension of less than 100 cfu/ml.

3. The method for aseptically detecting the crosslinked sodium hyaluronate gel for injection according to claim 1, wherein the preparation method of the hyaluronidase solution comprises the following steps: weighing a proper amount of hyaluronidase, dissolving in 100ml of 0.9% sterile sodium chloride solution, shaking to dissolve, and filtering with a sterile filter membrane of 0.22 μm in a sterile isolator after dissolving.

4. The method for aseptically measuring crosslinked sodium hyaluronate gel for injection according to claim 1, wherein in step A2, the crosslinked sodium hyaluronate gel for injection is extruded from a needle tube.

Technical Field

The invention relates to the field of microbial detection, in particular to a sterile detection method of cross-linked sodium hyaluronate gel for injection.

Background

In the method for detecting the sterility of the cross-linked sodium hyaluronate gel for injection in the prior art, a sample is directly inoculated, and the gel cannot be dissolved, so that microorganisms cannot be well detected, and false negative results are easily caused.

Disclosure of Invention

The invention aims to provide a method for aseptically detecting cross-linked sodium hyaluronate gel for injection, which has high accuracy and is easy to realize.

The invention provides a method for aseptically detecting cross-linked sodium hyaluronate gel for injection, which comprises the following steps:

a1: preparing a bacterial suspension or a spore suspension;

a2: preparation of a test solution: taking 90ml of hyaluronidase solution containing 300IU/ml, taking 10ml of crosslinked sodium hyaluronate gel for injection, putting the crosslinked sodium hyaluronate gel into the hyaluronidase solution, hydrolyzing at normal temperature, continuously shaking in the hydrolysis process, and shaking uniformly after the gel is completely hydrolyzed to obtain the hyaluronic acid gel;

a3: the detection method comprises the following steps:

a 1: preparation of test article groups: filtering 200ml of sample solution by using a dual disposable totally-enclosed bacteria collection culture device, adding 100ml of thioglycollate fluid medium (FTM) culture medium into one bacteria collection cup of the bacteria collection culture device after the completion, and adding 100ml of trypticase soy peptone liquid medium (TSB) culture medium into the other bacteria collection cup;

a 2: preparation of test article test group: performing the same operation with the test sample group, preparing 3 bacteria collection cups for each culture medium of a thioglycollate fluid medium (FTM) and a trypticase soy peptone liquid medium (TSB), respectively, totally 6 bacteria collection cups, transferring to a positive control detection room after completion, and respectively adding test bacteria less than l00 cfu; wherein, bacterial suspension or spore suspension of staphylococcus aureus, escherichia coli and clostridium sporogenes is added into a thioglycollate fluid medium (FTM) culture medium, and bacterial suspension or spore suspension of candida albicans, aspergillus niger and bacillus subtilis is added into a trypticase soy peptone liquid medium (TSB) culture medium;

a 3: preparation of positive control group: taking 3 cups of disposable sterile bacteria collection cups containing 100ml of thioglycollate fluid medium (FTM) and 100ml of trypticase soy peptone liquid medium (TSB) medium respectively, and adding test bacteria with the same amount as the bacteria added in the test group of the test sample to a positive control detection room; wherein, bacterial suspension or spore suspension of staphylococcus aureus, escherichia coli and clostridium sporogenes is added into a thioglycollate fluid medium (FTM) culture medium, and bacterial suspension or spore suspension of candida albicans, aspergillus niger and bacillus subtilis is added into a trypticase soy peptone liquid medium (TSB) culture medium;

a 4: preparation of negative control group: filtering 200ml of hyaluronidase solution containing 300IU/ml by using a dual disposable totally-enclosed bacteria collection culture device, adding 100ml of thioglycollate fluid medium (FTM) culture medium into one bacteria collection cup of the bacteria collection culture device after the filtration is finished, and adding 100ml of trypticase soy peptone liquid medium (TSB) culture medium into the other bacteria collection cup;

a 5: colony count confirmation group: test bacteria with the same amount as that added in the test group added with the test sample are taken and added into corresponding culture media, staphylococcus aureus, bacillus subtilis and escherichia coli are subjected to colony number confirmation by using a trypticase soytone agar medium (TSA), candida albicans and aspergillus niger are subjected to colony number confirmation by using a Sabouraud glucose agar medium (SDA), and clostridium sporogenes are subjected to colony number confirmation by using a thioglycollate fluid medium (FTM).

a 6: and (5) culturing.

Further, in step A1, Staphylococcus aureus, Escherichia coli, Clostridium sporogenes, Bacillus subtilis, Candida albicans, and Aspergillus niger are made into bacterial suspension or spore suspension less than 100 cfu/ml.

Further, the preparation method of the hyaluronidase solution comprises the following steps: weighing a proper amount of hyaluronidase, dissolving in 100ml of 0.9% sterile sodium chloride solution, shaking to dissolve, and filtering with a sterile filter membrane of 0.22 μm in a sterile isolator after dissolving. A

Further, in step a2, the cross-linked sodium hyaluronate gel for injection was extruded from the syringe.

By adopting the sterile detection method of the cross-linked sodium hyaluronate gel for injection, provided by the invention, hyaluronidase is adopted to dissolve a sample to be tested, so that the problem of false negative is avoided, direct inoculation is carried out, and microorganisms cannot be well detected due to the fact that the gel cannot be dissolved.

Detailed Description

In the description of the present invention, it is to be noted that those whose specific conditions are not specified in the examples are carried out according to the conventional conditions or the conditions recommended by the manufacturers. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.

The strains used in the following examples are as follows:

serial number	Name (R)	Strain numbering	Algebra	Preparation and use
					1	Staphylococcus aureus	CMCC(B)-26003	No more than 5 th generation	Prepared on the same day and used within 2 hours
2	Escherichia coli	CMCC(B)44102	No more than 5 th generation	Prepared on the same day and used within 2 hours
					3	Clostridium sporogenes	CMCC(B)64941	No more than 5 th generation	Prepared on the same day and used within 2 hours
4	Bacillus subtilis	CMCC(B)-63501	No more than 5 th generation	Prepared on the same day and used within 2 hours
					5	Candida albicans	CMCC(F)-98001	No more than 5 th generation	Prepared on the same day and used within 2 hours
6	Aspergillus niger	CMCC(F)98003	No more than 5 th generation	Prepared on the same day and used within 2 hours

The strain used for inoculation has good morphological characteristics and the number of passages cannot exceed 5.

The embodiment provides a method for detecting sterility of cross-linked sodium hyaluronate gel for injection, which comprises the following steps:

a1: preparing a bacterial suspension or a spore suspension;

a2: preparation of a test solution: taking 90ml of hyaluronidase solution containing 300IU/ml, taking 10ml of crosslinked sodium hyaluronate gel for injection, putting the crosslinked sodium hyaluronate gel into the hyaluronidase solution, hydrolyzing at normal temperature, continuously shaking in the hydrolysis process, and shaking uniformly after the gel is completely hydrolyzed to obtain the hyaluronic acid gel;

a3: the detection method comprises the following steps:

a 1: preparation of test article groups: filtering 200ml of sample solution by using a dual disposable totally-enclosed bacteria collection culture device, adding 100ml of thioglycollate fluid medium (FTM) culture medium into one bacteria collection cup of the bacteria collection culture device after the completion, and adding 100ml of trypticase soy peptone liquid medium (TSB) culture medium into the other bacteria collection cup;

a 2: preparation of test article test group: performing the same operation with the test sample group, preparing 3 bacteria collecting cups respectively for each culture medium of a thioglycollate fluid medium (FTM) and a trypticase soy peptone liquid medium (TSB), transferring to a positive control detection room after completing the preparation, and respectively adding test bacteria less than 100 cfu; wherein, bacterial suspension or spore suspension of staphylococcus aureus, escherichia coli and clostridium sporogenes is added into a thioglycollate fluid medium (FTM) culture medium, and bacterial suspension or spore suspension of candida albicans, aspergillus niger and bacillus subtilis is added into a trypticase soy peptone liquid medium (TSB) culture medium;

a 3: preparation of positive control group: taking 3 cups of disposable sterile bacteria collection cups containing 100ml of thioglycollate fluid medium (FTM) and 100ml of trypticase soy peptone liquid medium (TSB) medium respectively, and adding test bacteria with the same amount as the bacteria added in the test group of the test sample to a positive control detection room; wherein, bacterial suspension or spore suspension of staphylococcus aureus, escherichia coli and clostridium sporogenes is added into a thioglycollate fluid medium (FTM) culture medium, and bacterial suspension or spore suspension of candida albicans, aspergillus niger and bacillus subtilis is added into a trypticase soy peptone liquid medium (TSB) culture medium;

a 4: preparation of negative control group: filtering 200ml of hyaluronidase solution containing 300IU/ml by using a dual disposable totally-enclosed bacteria collection culture device, adding 100ml of thioglycollate fluid medium (FTM) culture medium into one bacteria collection cup of the bacteria collection culture device after the filtration is finished, and adding 100ml of trypticase soy peptone liquid medium (TSB) culture medium into the other bacteria collection cup;

a 5: colony count confirmation group: test bacteria with the same amount as that added in the test group added with the test sample are taken and added into corresponding culture media, staphylococcus aureus, bacillus subtilis and escherichia coli are subjected to colony number confirmation by using a trypticase soytone agar medium (TSA), candida albicans and aspergillus niger are subjected to colony number confirmation by using a Sabouraud glucose agar medium (SDA), and clostridium sporogenes are subjected to colony number confirmation by using a thioglycollate fluid medium (FTM).

a 6: culturing: : placing tryptone soy peptone liquid medium (TSB) of the test sample test group and the positive control group and Sa's dextrose agar medium (SDA) of the colony number confirmation group at 20-25 ℃ for culturing for 5 days, placing the thioglycolate fluid medium (FTM) of the test sample test group and the positive control group and the tryptone soy peptone agar medium (TSA) and the thioglycolate fluid medium (FTM) of the colony number confirmation group at 30-35 ℃ for culturing for 5 days, placing the tryptone soy peptone liquid medium (TSB) of the test sample group and the negative control group at 20-25 ℃ for culturing for 14 days, and placing the thioglycolate fluid medium (FTM) of the test sample group and the negative control group at 30-35 ℃ for culturing for 14 days.

In this example, the tryptic Soy peptone liquid Medium (TSB) of the test sample test group and the positive control group and the Sabourdon dextrose agar Medium (SDA) of the colony number confirmation group were incubated at 22 ℃ for 5 days, the thioglycolate fluid Medium (FTM) of the test sample test group and the positive control group and the tryptic Soy peptone agar Medium (TSA) and the thioglycolate fluid Medium (FTM) of the colony number confirmation group were incubated at 33 ℃ for 5 days, the tryptic Soy peptone liquid Medium (TSB) of the test sample group and the negative control group was incubated at 22 ℃ for 14 days, and the thioglycolate fluid Medium (FTM) of the test sample group and the negative control group was incubated at 33 ℃ for 14 days.

In step A1, Staphylococcus aureus, Escherichia coli, Clostridium sporogenes, Bacillus subtilis, Candida albicans, and Aspergillus niger are made into bacterial suspension or spore suspension less than 100 cfu/ml.

The preparation method of the hyaluronidase solution comprises the following steps: weighing a proper amount of hyaluronidase, dissolving in 100ml of 0.9% sterile sodium chloride solution, shaking to dissolve, and filtering with a sterile filter membrane of 0.22 μm in a sterile isolator after dissolving.

Wherein, in the step A2, the cross-linked sodium hyaluronate gel for injection is extruded from the needle tube.

As a result: the negative control group and the test sample group grow aseptically, the positive control group grows well, compared with the control group, the test bacteria of the test sample group grow well, the result shows that the test quantity of the test sample has no bacteriostasis or the bacteriostasis can be ignored under the test condition, the colony number of the colony number confirmation group meets the requirement and is not more than 100cfu/ml, and the sterility test of the test sample can be carried out according to the test method and the test condition.

By adopting the sterile detection method of the cross-linked sodium hyaluronate gel for injection provided by the embodiment of the invention, hyaluronidase is adopted to dissolve a sample to be tested, so that the problem of false negative is avoided, direct inoculation is carried out, and microorganisms cannot be well detected due to the fact that the gel cannot be dissolved.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.