Gene detection method and device
阅读说明:本技术 一种基因检测方法及其装置 (Gene detection method and device ) 是由 魏瑷琳 于 2020-07-20 设计创作,主要内容包括:本发明属于生物工程技术领域,尤其是涉及一种基因检测方法,包括以下步骤:检测准备、添加试剂、翻转和清洗,基因检测过程中使用到的装置包括箱体,所述箱体滑动连接有盖板,所述盖板固定连接有供液箱,所述箱体设有隔板,所述隔板可拆卸连接有多组培养皿,所述供液箱和培养皿配合设置,所述培养皿通过翻转机构和隔板活动连接,所述箱体固定连接有驱动电机,所述驱动电机的输出轴通过传动件活动连接有清洗机构,所述清洗机构和箱体滑动连接,所述清洗机构和翻转机构传动连接。本发明可实现培养皿的整体式自动添加试剂工作,降低工作人员手动操作的劳动强度,提高基因检测效率,同时装置具备自动清洁功能,适合范围进行推广。(The invention belongs to the technical field of biological engineering, and particularly relates to a gene detection method, which comprises the following steps: detect and prepare, add reagent, upset and wash, the device that uses in the gene testing process includes the box, box sliding connection has the apron, apron fixedly connected with liquid supply box, the box is equipped with the baffle, the baffle can be dismantled and be connected with the multiunit culture dish, liquid supply box and culture dish cooperation set up, the culture dish passes through tilting mechanism and baffle swing joint, box fixedly connected with driving motor, driving motor's output shaft has wiper mechanism through driving medium swing joint, wiper mechanism and box sliding connection, wiper mechanism and tilting mechanism transmission are connected. The invention can realize the integral automatic reagent adding work of the culture dish, reduce the labor intensity of manual operation of workers, improve the gene detection efficiency, and meanwhile, the device has the automatic cleaning function and is suitable for popularization in a range.)
1. A gene detection method is characterized by mainly comprising the following steps:
s1 detection preparation: stably placing a container for storing a gene sample to be detected through the device, and carrying out preparation work of gene detection;
s2 reagent addition: integrally and automatically adding a reagent into the stably placed gene sample by a mechanical means, and performing gene detection;
s3 turning: after the gene detection work of S2 is finished, the container for storing the gene sample is directly turned over by the device, so that the cleaning work of the container is facilitated;
s4 cleaning: the turned gene detection container is cleaned by rotation so as to facilitate subsequent detection work.
2. The utility model provides a gene detection device, its characterized in that, includes box (1), box (1) sliding connection has apron (2), apron (2) fixedly connected with liquid supply tank (3), box (1) is equipped with baffle (4), baffle (4) can be dismantled and be connected with multiunit culture dish (5), liquid supply tank (3) and culture dish (5) cooperation setting, culture dish (5) are through tilting mechanism (6) and baffle (4) swing joint, box (1) fixedly connected with driving motor (7), the output shaft of driving motor (7) has wiper mechanism (9) through driving medium (8) swing joint, wiper mechanism (9) and box (1) sliding connection, wiper mechanism (9) and tilting mechanism (6) transmission are connected.
3. The gene detecting device according to claim 2, wherein one end of the cover plate (2) is fixedly connected with a handle (21), the other end of the cover plate (2) is symmetrically provided with sliding blocks (22), the box body (1) is provided with a sliding groove (11), and the sliding blocks (22) are connected with the sliding groove (11) in a rolling manner through balls (221).
4. The gene detecting device according to claim 2, wherein the cover plate (2) is provided with a water distribution pipe (23), one end of the water distribution pipe (23) is communicated with the liquid supply tank (3), the other end of the water distribution pipe (23) is matched with the culture dish (5) through a rubber taper pipe (24), and the rubber taper pipe (24) is positioned right above the culture dish (5).
5. The gene detecting device according to claim 4, wherein the liquid supply tank (3) comprises a water supply tank (31) and a reagent tank (32), the water supply tank (31) and the reagent tank (32) are both communicated with the water distribution pipe (23) through a liquid conveying pipe (33), a water discharging pipe (12) is arranged at one end of the tank body (1) far away from the liquid supply tank (3), and the water discharging pipe (12) and the liquid conveying pipe (33) are both provided with a control valve (34).
6. The gene detecting device according to claim 2, wherein the partition board (4) is of a circular structure, the partition board (4) is provided with a plurality of sets of placing holes (41), the culture dish (5) is matched and connected with the placing holes (41), one end of the box body (1) close to the partition board (4) is provided with the heater (13), the other end of the box body (1) close to the partition board (4) is provided with the ultraviolet lamp (14), and the box body (1) is provided with the observation window (15).
7. The gene detecting device according to claim 2, wherein the turnover mechanism (6) comprises a rotating shaft (61), a gear ring (62) and a clamping member (63), the rotating shaft (61) is rotatably connected with the box body (1), one end of the gear ring (62) is fixedly connected with the rotating shaft (61), and the other end of the gear ring (62) is movably connected with the clamping member (63) through a first gear (64).
8. The gene detecting device according to claim 7, wherein the clamping member (63) comprises a guide rod (631), a sleeve (632) and a clamping block (633), one end of the guide rod (631) is fixedly connected with the first gear (64), the other end of the guide rod (631) is slidably connected with the sleeve (632) through a spring (634), the sleeve (632) is fixedly connected with the clamping block (633), the culture dish (5) is provided with a cross groove (51), and the clamping block (633) is in fit connection with the cross groove (51).
9. The gene detecting device according to claim 6, wherein the cleaning mechanism (9) comprises a support frame (91) and cleaning cotton (92), the cleaning cotton (92) is rotatably connected with the support frame (91) through a cleaning motor (93), and the support frame (91) is in threaded connection with the rotating shaft (61) through a threaded sleeve (94).
10. The gene detecting device according to claim 9, wherein the transmission member (8) comprises a second gear (81) and a rack (82) which are engaged with each other, the second gear (81) is fixedly connected with an output shaft of the driving motor (7), one end of the supporting frame (91) is fixedly connected with the rack (82), and the other end of the supporting frame (91) is slidably connected with the box body (1) through a telescopic rod (95).
Technical Field
The invention belongs to the technical field of biological engineering, and particularly relates to a gene detection method and a gene detection device.
Background
The gene detection is a technique for detecting DNA by blood, other body fluids or cells, and is a method for detecting DNA molecular information in cells of a detected person by a specific device after the peripheral venous blood or other tissue cells of the detected person are amplified, and analyzing whether the gene type, the gene defect and the expression function contained in the DNA molecular information are normal or not. Gene detection is one of the detection methods commonly used in bioengineering, but as the detection requirements of bioengineering are continuously improved, the defects of gene detection work in the actual operation process are urgently needed to be perfected.
Disclosure of Invention
The invention aims to solve the problems, and provides a gene detection method and a gene detection device which can automatically add a reagent to improve the detection efficiency, can realize automatic cleaning after gene detection is finished, and can quickly carry out secondary use on a culture dish.
In order to achieve the purpose, the invention adopts the following technical scheme:
a gene detection method mainly comprises the following steps:
s1 detection preparation: stably placing a container for storing a gene sample to be detected through the device, and carrying out preparation work of gene detection;
s2 reagent addition: integrally and automatically adding a reagent into the stably placed gene sample by a mechanical means, and performing gene detection;
s3 turning: after the gene detection work of S2 is finished, the container for storing the gene sample is directly turned over by the device, so that the cleaning work of the container is facilitated;
s4 cleaning: the turned gene detection container is cleaned by rotation so as to facilitate subsequent detection work.
Still adopted a gene detection device, the power distribution box comprises a box body, box sliding connection has the apron, apron fixedly connected with liquid supply box, the box is equipped with the baffle, the baffle can be dismantled and be connected with the multiunit culture dish, liquid supply box and culture dish cooperation setting, the culture dish passes through tilting mechanism and baffle swing joint, box fixedly connected with driving motor, driving motor's output shaft has wiper mechanism through driving medium swing joint, wiper mechanism and box sliding connection, wiper mechanism and tilting mechanism transmission are connected.
In the gene detection device, one end of the cover plate is fixedly connected with the handle, the other end of the cover plate is symmetrically provided with the sliding blocks, the box body is provided with the sliding groove, and the sliding blocks are in rolling connection with the sliding groove through the rolling balls.
In the gene detection device, the cover plate is provided with a water distribution pipe, one end of the water distribution pipe is communicated with the liquid supply tank, the other end of the water distribution pipe is matched with the culture dish through a rubber taper pipe, and the rubber taper pipe is positioned right above the culture dish.
In foretell gene testing device, the feed tank includes feed tank and reagent box, feed tank and reagent box all set up through transfer line and water distributor intercommunication, the one end that the feed tank was kept away from to the box is equipped with the drain pipe, drain pipe and transfer line all are equipped with control flap.
In foretell gene testing device, the baffle adopts circular structure, the baffle is equipped with the multiunit and places the hole, the culture dish with place the hole cooperation and connect, the one end that the box is close to the baffle is equipped with the heater, the other end that the box is close to the baffle is equipped with ultraviolet lamp, the box is equipped with the observation window.
In the above gene detection device, the turnover mechanism includes a rotating shaft, a toothed ring and a clamping member, the rotating shaft is rotatably connected with the box body, the rotating shaft at one end of the toothed ring is fixedly connected, and the other end of the toothed ring is movably connected with the clamping member through a first gear.
In the above gene detection device, the clamping member includes a guide rod, a sleeve and a clamping block, one end of the guide rod is fixedly connected with the first gear, the other end of the guide rod is slidably connected with the sleeve through a spring, the sleeve is fixedly connected with the clamping block, the culture dish is provided with a cross groove, and the clamping block is connected with the cross groove in a matching manner.
In the above gene detection device, the sleeve is provided with a limiting groove, the guide rod is provided with a limiting block, and the limiting block is slidably connected with the limiting groove.
In the above gene detection device, the cleaning mechanism comprises a support frame and cleaning cotton, the cleaning cotton is rotatably connected with the support frame through a cleaning motor, and the support frame is in threaded connection with the rotating shaft through a threaded sleeve.
In the above gene detection device, the transmission member includes a second gear and a rack that are engaged with each other, the second gear is fixedly connected with an output shaft of the driving motor, one end of the support frame is fixedly connected with the rack, and the other end of the support frame is slidably connected with the box body through a telescopic rod.
Compared with the prior art, the gene detection method and the device thereof have the advantages that:
1. according to the gene detection method and the device thereof, the liquid supply box is arranged, and the culture dish on the partition plate is matched for automatically adding the reagent, so that a worker does not need to manually add the reagent for detection in the gene detection work of the bioengineering, the gene detection efficiency is effectively improved, the labor intensity of the worker in operation is reduced, and the gene detection method and the device are suitable for popularization.
2. According to the gene detection method and the device, the cover plate is arranged, so that the device can perform sealing work in the gene detection process, and the liquid supply box is matched for integrally adding the reagent, so that the influence degree of foreign matters such as dust and bacteria in the air in the culture dish detection process on the gene detection is effectively reduced, the gene detection accuracy is improved, and the practicability of the device is improved.
3. According to the gene detection method and the device, the turnover mechanism is arranged, so that the culture dish automatically turns over after the gene detection work is finished, the cleaning mechanism is matched for cleaning the culture dish, workers do not need to clean in a disassembly and cleaning mode, the culture dish can be rapidly reused, the resource utilization of the device is effectively improved, the gene detection efficiency is improved, meanwhile, the contact between the workers and a gene sample is avoided, and the safety of the gene detection is improved.
Drawings
FIG. 1 is a schematic view of the flow structure of a gene detection method provided by the present invention;
FIG. 2 is a schematic view showing the external structure of a gene assaying device according to the present invention;
FIG. 3 is a schematic view of the internal structure of a gene assaying device according to the present invention;
FIG. 4 is an enlarged schematic view at A in FIG. 3;
FIG. 5 is an enlarged schematic view at B in FIG. 3;
FIG. 6 is a schematic view showing the structure of a partition plate in the gene assaying device according to the present invention.
In the figure, 1 a box body, 11 chutes, 12 water discharge pipes, 13 heaters, 14 ultraviolet lamps, 15 observation windows, 2 cover plates, 21 handles, 22 sliders, 221 balls, 23 water distribution pipes, 24 rubber taper pipes, 3 liquid supply tanks, 31 water supply tanks, 32 reagent tanks, 33 liquid conveying pipes, 34 control valves, 4 partition plates, 41 placement holes, 5 culture dishes, 51 cross grooves, 6 turnover mechanisms, 61 rotating shafts, 62 toothed rings, 63 clamping pieces, 631 guide rods, 632 sleeves, 633 clamping blocks, 634 springs, 64 first gears, 7 driving motors, 8 transmission pieces, 81 second gears, 82 racks, 9 cleaning mechanisms, 91 support frames, 92 cleaning cotton, 93 cleaning motors, 94 thread sleeves and 95 telescopic rods.
Detailed Description
The following examples are for illustrative purposes only and are not intended to limit the scope of the present invention.
As shown in FIG. 1, a gene detection method mainly comprises the following steps:
s1 detection preparation: stably placing a container for storing a gene sample to be detected through the device, and carrying out preparation work of gene detection;
s2 reagent addition: integrally and automatically adding a reagent into the stably placed gene sample by a mechanical means, and performing gene detection;
s3 turning: after the gene detection work of S2 is finished, the container for storing the gene sample is directly turned over by the device, so that the cleaning work of the container is facilitated;
s4 cleaning: the turned gene detection container is cleaned by rotation so as to facilitate subsequent detection work.
The detection method can directly clean the container by overturning and cleaning after the gene sample container finishes gene detection work, avoids manual collection and cleaning of workers, improves the work efficiency of the gene detection process, reduces the contact between the workers and the gene sample, and improves the safety of gene detection.
As shown in fig. 2-6, a gene detection device comprises a
2 fixedly connected with
Specifically,
More specifically, the
Baffle 4 adopts circular structure,
The
More specifically, clamping
The
Specifically,
More specifically,
The operating principle of the present invention will now be described as follows:
when the invention is used, a worker places the
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
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