阅读说明：本技术 一种测定青蒿精油皂抑菌强度的检测方法 (Detection method for determining antibacterial intensity of sweet wormwood essential oil soap ) 是由 孟连枝 郭宇鹏 贾鹏飞 张晶晶 王成龙 连津宣 朱和凤 解克伟 江红格 赵忠亮 于 2019-09-18 设计创作，主要内容包括：本发明涉及一种测定青蒿精油皂抑菌强度的检测方法,它包括以下步骤：步骤1、分别取100μL金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌、白色念珠菌四种菌液,并将其涂于不同的琼脂平皿板；步骤2、将青蒿精油皂溶解于无菌水制成100μg/μL的母液,然后用无菌水稀释成不同浓度的试剂；步骤3、取直径为6mm的滤纸片,贴于步骤1中的菌液平皿板上,各取100μL步骤2中不同浓度的试剂滴于滤纸片上；步骤4、将步骤3中的滤纸片分别置于37℃的温箱培养,分别在24h、48h、72h时观察是否出现抑菌圈并测量抑菌圈的直径大小,计算绝对抑菌率(%),并根据抑菌率的大小判定青蒿精油皂的抑菌强度；本发明具有操作简单快捷、可行性高、结果可靠的优点。(The invention relates to a detection method for determining the bacteriostatic intensity of sweet wormwood essential oil soap, which comprises the following steps: step 1, respectively taking 100 mu L of four bacterial liquids of staphylococcus aureus, escherichia coli, pseudomonas aeruginosa and candida albicans, and coating the bacterial liquids on different agar plates; step 2, dissolving the sweet wormwood essential oil soap in sterile water to prepare a mother solution with the concentration of 100 mu g/mu L, and then diluting the mother solution with the sterile water to obtain reagents with different concentrations; step 3, taking a filter paper sheet with the diameter of 6mm, pasting the filter paper sheet on the bacterial liquid plate in the step 1, and respectively taking 100 mu L of reagents with different concentrations in the step 2 and dripping the reagents on the filter paper sheet; step 4, respectively placing the filter paper sheets obtained in the step 3 in a 37 ℃ incubator for culture, observing whether an inhibition zone appears or not at 24 hours, 48 hours and 72 hours, respectively, measuring the diameter of the inhibition zone, calculating an absolute inhibition rate (%), and judging the inhibition intensity of the sweet wormwood essential oil soap according to the inhibition rate; the method has the advantages of simple and quick operation, high feasibility and reliable result.)

1. A detection method for determining the bacteriostatic intensity of sweet wormwood essential oil soap is characterized by comprising the following steps:

step 1, respectively taking 100 mu L of four bacterial liquids of staphylococcus aureus, escherichia coli, pseudomonas aeruginosa and candida albicans, and coating the bacterial liquids on different agar plates;

step 2, dissolving the sweet wormwood essential oil soap in sterile water to prepare a mother solution with the concentration of 100 mu g/mu L, and then diluting the mother solution into reagents with different concentrations by using the sterile water according to the gradient of 0.5%, 1%, 2%, 3%, 5% and 10%;

step 3, taking a filter paper sheet with the diameter of 6mm, pasting the filter paper sheet on the bacterial liquid plate in the step 1, and respectively taking 100 mu L of reagents with different concentrations in the step 2 and dripping the reagents on the filter paper sheet;

and 4, respectively placing the filter paper sheets obtained in the step 3 in a 37 ℃ incubator for culture, observing whether an inhibition zone appears or not and measuring the diameter of the inhibition zone at 24h, 48h and 72h, respectively, setting 3 parallel filter paper sheets in each group, taking an average value, calculating the absolute inhibition rate (%), and judging the inhibition intensity of the artemisia apiacea essential oil soap according to the inhibition rate.

2. The detection method for determining the bacteriostatic intensity of the artemisia apiacea essential oil soap according to claim 1, which is characterized in that: the concentrations of the four bacteria liquids in the step 1 are set as fixed values.

Technical Field

The invention belongs to the technical field of biological detection, and particularly relates to a detection method for determining the bacteriostatic intensity of sweet wormwood essential oil soap.

Background

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provides the detection method for determining the bacteriostatic strength of the artemisia apiacea essential oil soap, which is simple and rapid to operate, high in feasibility and reliable in result.

The purpose of the invention is realized as follows: a detection method for determining the bacteriostatic intensity of sweet wormwood herb essential oil soap comprises the following steps:

step 1, respectively taking 100 mu L of four bacterial liquids of staphylococcus aureus, escherichia coli, pseudomonas aeruginosa and candida albicans, and coating the bacterial liquids on different agar plates;

step 2, dissolving the sweet wormwood essential oil soap in sterile water to prepare a mother solution with the concentration of 100 mu g/mu L, and then diluting the mother solution into reagents with different concentrations by using the sterile water according to the gradient of 0.5%, 1%, 2%, 3%, 5% and 10%;

step 3, taking a filter paper sheet with the diameter of 6mm, pasting the filter paper sheet on the bacterial liquid plate in the step 1, and respectively taking 100 mu L of reagents with different concentrations in the step 2 and dripping the reagents on the filter paper sheet;

and 4, respectively placing the filter paper sheets obtained in the step 3 in a 37 ℃ incubator for culture, observing whether an inhibition zone appears or not and measuring the diameter of the inhibition zone at 24h, 48h and 72h, respectively, setting 3 parallel filter paper sheets in each group, taking an average value, calculating the absolute inhibition rate (%), and judging the inhibition intensity of the artemisia apiacea essential oil soap according to the inhibition rate.

The concentrations of the four bacteria liquids in the step 1 are set as fixed values.

The invention has the beneficial effects that: the method comprises the steps of respectively dripping the sweet wormwood essential oil soap solutions with different concentrations on four different bacteria liquid filter paper sheets, respectively placing the four different bacteria liquid filter paper sheets in a warm box for culturing for a period of time, observing and measuring the diameter of a bacteriostatic ring, calculating the bacteriostatic rate according to the measurement result, and judging the bacteriostatic intensity of the sweet wormwood essential oil soap according to the bacteriostatic rate; the invention establishes a method system for measuring the bacteriostatic strength of the sweet wormwood essential oil soap, and has the advantages of simple and quick operation, high feasibility and reliable result.

Detailed Description

The present invention will be further described with reference to the following examples. The present embodiments are to be considered as illustrative and not restrictive, the scope of the invention being indicated by the claims, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.