阅读说明：本技术 用于过重女性患者的基于屈螺酮的避孕药 (Drospirenone-based contraceptive for overweight female patients ) 是由 D·德鲁恩 C·布瓦耶-茹贝尔 P·佩兰 于 2016-06-23 设计创作，主要内容包括：以至少3mg的含量包含于每日活性剂量单元中的屈螺酮作为唯一的避孕成分用作肥胖女性患者的避孕药的用途。(Use of drospirenone as sole contraceptive ingredient contained in a daily active dosage unit in an amount of at least 3mg as contraceptive agent for obese female patients.)

1. A contraceptive kit comprising one or more packaging units, wherein each packaging unit comprises 21-28 daily active dose units, wherein:

a) the drospirenone content of each daily active dosage unit is at least 3mg, free of estrogen, and

b) each daily active dosage unit comprises drospirenone in a form such that:

(i) not more than 50% of the drospirenone originally present in said daily active dosage unit is dissolved within 30 minutes, and

(ii) at least 50% of said drospirenone is dissolved in a period of from 3 hours to 4 hours,

when the daily active dose is subjected to an in vitro dissolution test according to USP XXIII paddle method, the percentage of drospirenone is correlated to the amount of drospirenone initially present in said daily active dose unit.

2. A contraceptive kit according to claim 1, wherein the drospirenone content in each daily active unit dose is from 3.5mg to 4.5 mg.

3. A contraceptive kit according to claim 1, wherein the one or more packaging units further comprise 1-7 daily dosage units of a pharmaceutically acceptable placebo.

4. A contraceptive kit according to claim 1, wherein each packaging unit comprises 24 daily active dosage units.

5. A contraceptive kit according to claim 4, wherein each packaging unit comprises 4 daily placebo dosage units.

6. A contraceptive kit according to any one of claims 1 to 5, wherein the drospirenone is in crystalline form.

7. A contraceptive kit according to any one of claims 1 to 5, wherein the drospirenone is in non-micronized form.

8. A contraceptive kit according to any one of claims 1 to 5, wherein d of the drospirenone is₅₀Less than 70 μm.

9. A contraceptive kit according to any one of claims 1 to 5, wherein the drospirenone is in particulate form.

10. A contraceptive kit according to claim 9, wherein the drospirenone in particulate form has a specific surface area of 2,000cm²/g-8,500cm²/g。

11. Use of drospirenone for the preparation of a contraceptive kit according to any one of claims 1 to 10 which results in a reduction of the number of days of bleeding events in female patients.

Technical Field

The present invention relates to the field of contraceptive compositions and methods, particularly for overweight female patients, including overweight or obese female patients.

Background

In the general population, adult overweight (i.e., overweight and obese) is highly increasing over time.

The World Health Organization (WHO) has established a body mass standard status classification based on Body Mass Index (BMI) where the range of adults is as follows:

-characterized by a BMI of less than 18.5kg/m²The overweight condition of (1);

-characterized by a BMI of 18.5kg/m²-24.9kg/m²Normal weight state of (a);

-characterized by a BMI of 25.0kg/m²-29.9kg/m²An overweight condition of (a); and

-characterized by a BMI of 30.0kg/m²Or a higher obese state.

Based on the latest OECD hygiene statistics reports, a large proportion of adults in the European Union countries (53%) are Overweight or obese, with obese adults accounting for about 17% of the adult population (OECD/European Union (2014), "overhead and objective among adults," in Health at a Glance: Europe 2014, OECD Publishing). In the United states, 64% of women are overweight or obese, with 36% being obese (Flegal et al, Journal of the American Medical Association.2012; 307(5): 491-97). Obesity is a known risk factor for a number of health problems, including hypertension, high cholesterol, diabetes, cardiovascular disease, and some forms of cancer.

The incidence of accidental pregnancy is comparable to overweight or obesity. About 40% of all pregnancies worldwide are unexpected, with 43% in Europe and 51% in North America (Sedgh G. et al students in Family Planning 2014; 45(3): 301-314). Obese women, especially those with complications, have a higher risk of pregnancy related complications. It is therefore particularly important to avoid accidental pregnancy in these women (Cedergren MI. Obstet. Gynecol.2004; 103: 219-24).

The best way to prevent accidental pregnancy is to provide adequate contraception with a safe and well tolerated option.

Hormonal contraceptives are one of the most widely used contraceptive methods in the world and have the widest geographical distribution of any method. Hormonal contraceptives are also the most common form of reversible female contraception in the developed world (United nations. department of Economic and Social affaises-position division. world contextual Patterns 2013). Among the hormonal contraceptives, a combined contraceptive (pill, vaginal ring or transdermal patch) or a progestogenic contraceptive alone (progestogenic pill alone, injection, implant) can be cited.

A combined hormone contraceptive comprises a combination of progestagen and estrogenic components.

A number of pharmacological effects contribute to the contraceptive effect of progestagens. These include inhibition of ovulation by inhibiting the function of the hypothalamic-pituitary-ovarian axis; altering the subsequent pituitary surge of luteinizing hormone and follicle stimulating hormone. It also includes slowing the transit of the ovum through the fallopian tube, which limits the time available for fertilization; thickening cervical mucus, which prevents sperm transport; and inhibition of activation of the sperm enzymes required for egg penetration. The primary purpose of estrogen is to provide adequate circulatory (bleeding) control; it also inhibits the release of follicle stimulating hormone and thus inhibits follicular development.

One of the side effects of combination oral contraceptives is the increase of Venous Thromboembolic (VTE) risk to two clinical features: deep vein thrombosis or pulmonary embolism. 10000 women of child-bearing age per year suffer from venous thromboembolic disease, and the incidence is increased three to five times in women using combined hormonal contraceptives. In women of reproductive age, combined hormone contraception remains the most important factor for the development of VTE and in most cases the only factor causing the disease (Blanco-Molina MA et al, Progestin-only conjugation and venous thrombosis, Thrombosins research.2012; 129: e257-e 262).

Obesity is an independent risk factor for the development of Venous Thromboembolism (VTE) (Abdollahi M. et al Thromb. Haemost. 2003; 89(3): 493-. The use of combination oral contraceptives is associated with an increased risk of VTE in obese patients. A population-based case-control study conducted in the netherlands has demonstrated this increased risk. In women who do not use oral contraceptives, the risk for overweight women is increased 2.5 times and the risk for obese women is increased 3.0 times compared to normal weight women who do not use oral contraceptives. The VTE risk of overweight oral contraceptive users was increased 11.6 times and that of obese oral contraceptive users 23.8 times relative to non-users with normal BMI (Murthy AS. Semin reproduced Med.2010 Mar; 28(2): 156-63; Pomp ER et al Br J Haematol 2007; 139: 289) 296.).

The estrogenic component of contraceptive formulations is believed to be responsible for the thrombolytic effect of combined hormone contraception.

According to the guidelines of the world health organization and the American center for disease control and prevention ("guidelines on medical eligibility standards for contraceptive use"), obese, hypertonic, smoking or diabetic women aged 35 years and older are discouraged from using a combination oral contraceptive. Progestogen contraception alone in all manner is advocated even for women with high VTE risk, such as those with genetic thrombophilia, history of estrogen-induced venous thromboembolic Disease, or history of recurrent venous thromboembolic Disease (Centers for Disease Control and prognosis. us medical eligibility criterion for concentrating use. mmwr Early Release 2010; 59:1-86.Department of productive Health. medical eligibility criterion for concentrating use. who preference, 2009).

Recall that progestogenic contraception (POC) alone relies on maintaining high levels of d-progesterone in the female body to inhibit the secretion of follicle stimulating hormone and luteinizing hormone. POC treatment results in the absence of any new follicular development.

Among the POC contraceptive formulations, a large number of progestogenic molecules may be mentioned which have proven effective as active ingredients in contraceptives, such as norethindrone, norethindrone diacetate, levonorgestrel, desogestrel, ethinylestradiol, drospirenone or long-acting megestrol acetate (DMPA).

With regard to progestin-alone contraception, a systematic review and meta-analysis from Mantha evaluated VTE risk in progestin-alone contraceptive users without identifying a significant risk of venous thromboembolism associated with contraception using progestin alone (Mantha S et al, BMJ 2012; 345: e 4944). In a recent study, Lidegaard analyzed more than 29000 women for a third generation progestogen-only bolus (desogestrel) and did not show any increase in VTE risk associated with its use (adjusted venous thromboembolic event rate 0.64 (95% confidence interval 0.29-1.42)) (Lidegaard O. et al, 2001-9.BMJ 2011; 343: d 6423).

Therefore, progestagenic contraceptives alone should be the best choice for VTE-risk women and therefore should be a safe choice for overweight or obese women.

However, a major known disadvantage of progestagenic contraceptives alone is the alteration of the bleeding characteristics, resulting in breakthrough bleeding or spotting during hormonal treatment.

It is well known that irregular bleeding is one of the important reasons for stopping contraception, which increases the risk of accidental pregnancy. Women who stop taking oral contraceptives often fail to substitute another contraceptive or take less effective measures. In fact, one study showed that 23% of 6% of users who stopped oral contraceptives due to irregular bleeding had an intentional pregnancy outside (Rosenberg MJ et al, JReprod. Med. 1995; 40 (5); 355-.

The incidence of breakthrough bleeding in overweight or obese women using hormonal contraception is unknown. Retrospective analysis of data from a study evaluating 2893 women (613 overweight or obese) using two different formulations of low-dose oral contraceptives revealed that breakthrough bleeding rates did not differ between weight classes (Hampton RM et al, containment 2008; 77(6): 415-419). Another study showed that there was no overall difference in the total number of days of bleeding or spotting during the use of the contraceptive pessary Nuvaring (eton/ethinyl estradiol) (normal BMI:8.7 + -SD 7.3 days, obese: 7.7 + -SD 6.0 days p ═ 0.64) (Dragoman M. et al, content.2013 Apr; 87(4): 432-.

Irregular menstrual flow and frequency and weight gain are well documented side effects of POC treatments such as DMPA, a long acting reversible progestogenic contraceptive drug injected every 3 months (Harel Z et al. additives' responses for and experience after administration of the long-acting contraceptive delivery-success and normal. J advanced Health 1996; 19(2): 118-23; kaunintz AM. injectable contraceptive responsive acid delivery: update U.S. environmental benefits. J. intrinsic J. fertility metals 1998; 43(2): 73-83; Keder et al. compact with contraceptive efficacy, therapeutic delivery: 10. curative J. curative et al. 1997, sodium delivery, 1998; 43. 73-83; curative et al. curative effect: 10. curative et al. delivery patent No. 1997, 5. curative No. 5. curative delivery, J. curative No. 3. curative effect, 1998; promoter J. curative No. 5. curative effect: 3. delivery, 5. curative delivery, J. curative effect. delivery, 1. curative No. 5. curative delivery, 2. delivery, application No. 3. curative No. 5. curative effects; 419-28 in 13 (4); global planning of case exchange 1998; 3(1):4).

The only experimental study related to POC contraception based on DMPA seems to indicate a lower risk of excessive bleeding in overweight women (Connor et al; J.Am. Borad fam. practice.; 2002, vol.15(1):7-10) or obese women (Belsey et al; Contraception.1988 Aug; 38(2): 243-57). Connor et al (2002) show that women who exhibit overweight or obesity are at lower risk of developing increased or excessive bleeding when treated with DMPA with POC compared to their height-weight proportional counterparts. Connor et al (2002) are concerned with physiological effects including excessive flow, excessive bleeding, persistent bleeding, and persistent flow (collectively referred to herein as "excessive bleeding") and effects including increased spotting, increased flow, deeper menses, and increased menstrual duration (collectively referred to herein as "increased bleeding"). The comparison disclosed by Connor et al (2002) only relates to unique, overall bleeding parameters including "excessive bleeding" and "increased bleeding".

However, Connor et al do not relate to the possible effect of DMPA on the duration of a bleeding event, in particular the number of days on which bleeding or spotting occurs.

However, as is evident from the Connor et al study, women who are overweight or obese still have undesirable spotting and bleeding.

Still further, reference may also be made to a study published by Casey et al (2013, containment, Vol.87:370-374) which relates to etonogestrel-based POC contraceptives under the form of subcutaneous implants (ESI). Casey et al (2013) show that obese women are less likely to remove ESI implants because of bleeding irregularities than non-obese women.

As will be appreciated from the prior art knowledge discussed above, each of the known progestogen candidate molecules intended to formulate POC contraceptive formulations suitable for a particular administration regimen may behave differently, including their effect on various bleeding episodes.

Finally, it is generally known in the art that POC treatment is also often associated with weight gain, which may become a challenge for overweight women, in particular overweight women, especially obese women, which weight gain effect may increase the occurrence of diseases associated with overweight and/or obesity, such as, for example, coronary heart disease, hypertension, stroke, type 2 diabetes, dyslipidemia, metabolic syndrome, cancer, osteoarthritis, sleep apnea, obesity hypoventilation syndrome, infertility, gallstones, gout.

In particular, a systematic review of the assessment of Curtis using progestagen contraceptives alone in obese women indicates that adolescent DMPA users who are obese or overweight will gain more weight than normal weight users (Curtis KM. et al. Women are often challenged to contraception because of weight gain. Many women will stop their contraception due to this perceived side effect. In fact, studies with oral contraceptive users have found that the perception of weight gain is one of the important reasons why women in the United states stop contraception (Rosenberg M.weight change with oral contraceptive use and during the mental cycle: results of life measures. content 1998; 58: 345-9).

Thus, due to the above disadvantages, undesired bleeding or spotting, weight gain occurs, overweight women, especially overweight women and especially obese women are likely to break away from POC treatment, increasing their chance of accidental pregnancy.

Accordingly, there is a need to provide safe and effective contraceptive formulations for overweight women (encompassing both overweight and obese women), particularly to provide well-tolerated contraceptive formulations that exhibit improved patient compliance.

Brief description of the invention

The present invention relates to the use of Drospirenone (DRSP) as sole contraceptive ingredient in an amount of at least 3mg contained in a daily active dosage unit as contraceptive agent for overweight female patients, including overweight female patients as well as obese female patients.

The invention also relates to the use of drospirenone as sole contraceptive ingredient for the preparation of a contraceptive composition for overweight female patients, including overweight female patients as well as obese female patients.

The invention also relates to the use of drospirenone for the preparation of a contraceptive formulation that results in a reduction in the number of days of bleeding episodes in overweight female patients, including overweight female patients as well as obese female patients. A general and substantial reduction in the number of days of bleeding episodes as compared to female patients who are not overweight is throughout the present specification. The contraceptive formulation comprising drospirenone as the sole contraceptive ingredient is described in detail in the embodiments throughout this specification.

In another aspect, the invention also relates to a method comprising administering a pharmaceutical composition comprising drospirenone in an amount of at least 3mg to overweight female patients, including overweight female patients as well as obese female patients.

The present invention relates to a method comprising administering a pharmaceutical composition comprising drospirenone in an amount of at least 3mg to overweight female patients, including overweight female patients as well as obese female patients, wherein said pharmaceutical composition allows a daily dosing regimen of 28 days, and wherein the initial administration of drospirenone after establishing its contraceptive effect in the patient, the patient may skip up to 4 doses within a daily dosing regimen cycle of 28 days.

The invention also relates to the use of drospirenone for the preparation of a contraceptive formulation that results in a generally substantial reduction in the number of days of bleeding episodes in female patients, particularly overweight female patients, including obese female patients. A general and substantial reduction in the number of days of bleeding episodes as compared to female patients who are not overweight is throughout the present specification. The contraceptive formulation comprising drospirenone as the sole contraceptive ingredient is described in detail in the embodiments throughout this specification.

The present invention also relates to the use of drospirenone for the preparation of a contraceptive kit which results in a generally substantial reduction in the number of days of bleeding episodes in female patients, wherein said contraceptive kit comprises one or more packaging units, wherein each packaging unit comprises 21-28 active daily dosage units, wherein:

a) each daily active dosage unit comprising drospirenone in an amount of at least 3mg, being free of estrogen, and

b) the single daily active dosage unit is adapted to provide a pharmacokinetic profile of drospirenone when administered orally in the fasted state having:

(i) average t_maxIs 2.2h to 6h and

(ii) average C_maxLess than about 30ng/ml, and

(iii) optional mean AUC_{0h-t last}At least about 300ng h/ml.

C_maxAnd t_maxThe values refer to the maximum DRSP plasma concentration and the time to reach it, respectively, after oral administration of a single daily dosage unit of the subject composition comprising DRSP. In other words, C_maxAnd t_maxRefers to the peak plasma drospirenone concentration observed following oral ingestion of a single daily dosage unit of the subject composition.

AUC_{0h-t last}Corresponding to the interval [0h-t last]The area obtained by integrating the plasma concentration of endolospirone with time, point "0 h" means the oral intake of a single daily dosage unit of the subject composition, point "tinal" means the plasma concentration of DRSPThe last time can be quantified.

DRSP plasma concentrations can be determined by well known methods. For example, a suitable quantification method includes extraction of DRSP from human plasma followed by quantification using liquid chromatography in combination with tandem mass spectrometry.

In one embodiment, one skilled in the art can use the assay described by Kirk et al (Rapid Communication in Mass Spectrometry, 2006; 20: 1247-. The method comprises the step of derivatizing drospirenone with Girard P hydrazine solution to increase the reaction of DRSP during subsequent MS analysis. The method is generally suitable for quantifying DRSP in a concentration range of about 0.25 to about 100ng/ml in human EDTA plasma.

As used herein, mean AUC_{0h-t last}Average of C_maxAnd average t_maxMeans the arithmetic mean of the individual pharmacokinetic data determinations obtained from a group of healthy female volunteers of child bearing age who had been administered orally one daily dosage unit of a composition comprising drospirenone a single time. The group of healthy female volunteers may contain enough females to provide statistically confident pharmacokinetic results. Preferably, the group comprises at least ten healthy women of child bearing age.

As used herein, a healthy women of fertile age refers to a pre-menopausal caucasian woman of 18-40 years old, of normal weight and without health problems, in particular without metabolic, renal, hepatic or gynecological diseases.

The present invention also relates to the use of drospirenone for the preparation of a contraceptive kit resulting in a reduction of the number of days of bleeding events in a female patient, wherein said contraceptive kit comprises one or more packaging units, wherein each packaging unit comprises 21-28 active daily dosage units, wherein:

(a) the drospirenone content of each daily active dosage unit is at least 3mg, free of estrogen, and

(b) each daily active dosage unit comprises drospirenone in a form such that:

(i) not more than 50% of the drospirenone originally present in said daily active dosage unit is dissolved within 30 minutes, and

(ii) when the daily active dose is subjected to an in vitro dissolution test according to USP XXIII paddle method, at least 50% of said drospirenone is dissolved over a period of from 3 hours to 4 hours, the percentage drospirenone being relative to the amount of drospirenone originally present in said daily active dose unit.

Drawings

FIG. 1: a graph showing the correlation between Body Mass Index (BMI) versus body weight change (kg) for women undergoing DRSP-based contraceptive therapy. Body weight change was measured by subtracting body weight at visit 6 (day 24 ± 2 of cycle 13) from body weight at screening. Any negative results indicate weight loss. BMI less than 30kg/m²The (left) women did not show any mean weight change, whereas the BMI was 30kg/m²Or higher (right) women show slight but significant weight changes during contraceptive therapy.

FIG. 2: a graph showing the correlation between Body Mass Index (BMI) versus heart rate change for women undergoing DRSP-based contraceptive therapy. Heart rate change was measured by subtracting the heart rate at visit 6 (day 24 ± 2 of cycle 13) from the heart rate at screening. Any negative results indicate a higher heart rate during contraceptive therapy. BMI less than 30kg/m²(left) women showed a slight but significant increase in heart rate during contraceptive therapy, with a BMI of 30kg/m²Or higher (right) women do not show any heart rate variation during contraceptive therapy.

Detailed Description

The inventors have recently developed a new contraceptive kit and a new contraceptive pharmaceutical composition based on drospirenone in crystalline and non-micronized form (WO 2012/000981). DRSP is a fourth generation progestogen which is derived from spironolactone and has pharmacological characteristics which mimic natural progesterone.

DRSP has no androgenic, glucocorticoid and antiglucocorticoid activity, but does have powerful anti-mineralocorticoid and anti-androgenic properties. It has been shown that an oral daily dose of at least 3mg of DRSP inhibits ovulation within a single treatment cycle of 21 days. The combination of 3mg DRSP/30 μ g ethinylestradiol provided a reasonable contraceptive safety margin by inhibiting ovulation and low frequency follicle maturation (Rosenbaum et al, 2000, The European Journal of restriction and reproduction Health Care,5, 16-24).

In contrast to other DRSP-based contraceptive formulations on the market, DRSP-based contraceptive formulations according to WO2012/000981 rely on a slow dissolution rate of DRSP in vitro.

Furthermore, the DRSP-based contraceptive formulation according to WO2012/000981 shows characteristic pharmacokinetic characteristics. In particular, a single daily active dose unit comprising a DRSP content of at least 3mg is adapted to provide a DRSP pharmacokinetic profile having:

average t_maxIs 2.2h to 6h and

average C_maxLess than about 30 ng/ml.

It has been shown that WO2012/000981 discloses contraceptive pharmaceutical compositions based on Drospirenone (DRSP) in crystalline and non-micronized form suitable for administration to a BMI below 30kg/m²To a female patient.

Within the scope of the following, the term is used for the standardized description of bleeding and/or spotting in female patients for contraceptive therapy (Mishell et al, 2007, containment, 75(1): 11).

The term "bleeding" or the expression "bleeding event" is intended to mean blood loss requiring the use of a tampon, pad or pad.

The term "spotting" or the expression "spotting event" is intended to mean minimal blood loss without the need to use any type of protective measures.

The expression "onset of bleeding and/or spotting" is intended to mean the number of days of bleeding and/or spotting delimited at either end of a two-day bleed or spotting.

In some embodiments, the term "bleeding" may also encompass "spotting".

As shown in the examples herein, overweight women treated with DRSP-based POC formulations described herein (i.e., BMI of 25 kg/m)²Or above) of a non-overweight female (i.e., a BMI of 24.9 kg/m) undergoing the same contraceptive therapy²Or less) is reduced by about 22.5% during treatment cycles 2-4, and (ii) is compared to a non-overweight female undergoing the same contraceptive treatment (i.e., a BMI of 24.9 kg/m)²Or less) is reduced by about 27.7% during treatment cycles 2-6, (iii) is compared to a non-overweight female undergoing the same contraceptive treatment (i.e., a BMI of 24.9 kg/m)²Or less) is reduced by about 30.8% during treatment cycles 2-9, (iv) is compared to a non-overweight female undergoing the same contraceptive treatment (i.e., a BMI of 24.9 kg/m)²Or less) by about 23.1% during treatment cycles 5-7, and (v) by a non-overweight female undergoing the same contraceptive treatment (i.e., a BMI of 24.9 kg/m)²Or less) by about 22.5% during treatment cycles 7-9. The observed differences were statistically significant (p) using Wilcoxon-rank sum test (p)<0.01)。

As further shown in the examples herein, overweight women (i.e., BMI of 25 kg/m)²Or above women) the average number of days of bleeding episodes (i) during treatment cycles 2-4, each treatment cycle does not exceed 13%; (ii) during treatment cycles 2-6, no more than 11% of each treatment cycle; (iii) during treatment cycles 2-9, treatment cycles 5-7, or treatment cycles 7-9, no more than 10% of each treatment cycle is present.

As used herein, a "treatment cycle" encompasses a 28-day course of treatment, wherein the treatment comprises administration of 21-28 consecutive daily doses of the active test product tablet and may comprise administration of up to 7 daily doses of the placebo tablet.

In a preferred embodiment, the treatment cycle comprises administering 24 consecutive daily doses of tablets of the active test product followed by 4 consecutive days of administration of placebo tablets.

As used herein, "number of bleeding events per treatment cycle" encompasses the number of days with one or more bleeding episodes within a treatment cycle, regardless of the period of the cycle, i.e., active test product or placebo.

As shown in the examples herein, overweight women treated with DRSP-based POC formulations described herein (i.e., BMI of 25 kg/m)²Or above) the number of bleeding episodes per treatment cycle and progressionNon-overweight women of the same contraceptive treatment (i.e., BMI of 24.9 kg/m)²Or below) is always reduced by at least 5.0% over any of the cycles of treatment studied.

As used herein, a "at least 5%" reduction in the number of days of bleeding events per treatment cycle encompasses a reduction of at least 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29% and 30%.

For example, the mean percent reduction in the number of bleeding episodes per treatment cycle in overweight women treated with DRSP-based POC formulations over 3 consecutive treatment cycles can be assessed, which can be 15% to 30%, preferably 20% to 25%, more preferably 22% to 24% compared to non-overweight women on the same contraceptive treatment.

It will be appreciated that the percentage reduction in the number of bleeding event days per treatment cycle in overweight women may vary depending on parameters such as, for example, initial weight, age, time elapsed before treatment began.

As further shown in the examples herein, obese women treated with the DRSP-based POC formulations described herein (i.e., a BMI of 30 kg/m)²Or above) of bleeding episodes per treatment cycle (i) of a non-obese female undergoing the same contraceptive therapy (i.e., a BMI of 29.9 kg/m)²Or less) is reduced by about 60.1% during treatment cycles 2-4, (ii) is compared to a non-obese female undergoing the same contraceptive treatment (i.e., a BMI of 29.9 kg/m)²Or less) is reduced by about 67.9% during treatment cycles 2-6, (iii) is compared to a non-obese female undergoing the same contraceptive treatment (i.e., a BMI of 29.9 kg/m)²Or less) is reduced by about 69.7% during treatment cycles 2-9, (iv) is compared to a non-obese female undergoing the same contraceptive treatment (i.e., a BMI of 29.9 kg/m)²Or less) is reduced by about 48.1% during treatment cycles 5-7 and (v) is compared to a non-obese female undergoing the same contraceptive treatment (i.e., a BMI of 29.9 kg/m)²Or less) by about 62.0% during treatment cycles 7-9.

The observed differences were statistically significant (p <0.01) using Wilcoxon-rank sum test.

As further shown in the examples herein, obese women (i.e., BMI of 30 kg/m)²Or above females) has not more than about 7% of the average number of days of bleeding episodes (i) during treatment cycles 2-4, during treatment cycles 5-7; (ii) no more than about 5% during treatment cycles 2-6, treatment cycles 2-9, and treatment cycles 7-9.

Furthermore, as further shown in the examples herein, drospirenone based compositions may benefit obese women compared to other POC based contraceptive compositions, as a significant reduction in the number of days within a cycle bleeding or spotting event is observed.

Thus, as shown in the examples herein, obese women treated with the DRSP-based POC formulations described herein (i.e., a BMI of 30 kg/m)²Or above) with non-obese women undergoing the same contraceptive therapy (i.e., a BMI of 29.9 kg/m)²Or below) is always reduced by at least 10.0% or more during any of the cycles of treatment studied.

As used herein, a reduction in the number of days of bleeding episodes per treatment cycle of "at least 10%" encompasses a reduction of at least 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49% and 50%.

The mean percent reduction in the number of days of bleeding episodes per treatment cycle in obese women treated with DRSP-based POC formulations over 3 consecutive treatment cycles, as compared to non-obese women on the same contraceptive therapy, can be further evaluated and can range from 30% to 75%, preferably from 40% to 75%, more preferably from 45% to 65%.

The reduction in days of bleeding or spotting shown by the inventors can be beneficial to overweight women (including overweight and obese women) and considerably improve their comfort of life while providing compliant contraceptive therapy and increased contraceptive therapy efficiency.

In addition to a reduction in the number of days of bleeding or spotting, oral administration of DRSP-based POC therapy according to WO2012/000981 to overweight women, particularly obese women, may also provide benefits in terms of weight change and in terms of resting heart rate change, as shown in the examples herein.

These unexpected observations could not be expected from previous clinical evaluations disclosed in WO 2012/000981.

In fact, when administered to overweight women, especially obese women, such DRSP-based contraceptive compositions resulted in a significant average weight loss and no change in resting heart rate compared to women from the general population (no effect on weight change and a slight significant increase in resting heart rate observed during treatment).

Thus, DRSP-based contraceptive treatments according to the present invention allow for inclusion of obese women, which cover a Body Mass Index (BMI) of 30kg/m²Or above) better follow prescribed contraceptive therapy, thereby reducing the risk of weaning from said contraceptive therapy and thus increasing the risk of accidental pregnancy.

Thus, in some embodiments, the present invention relates to the use of drospirenone as sole active ingredient contained in a daily active dosage unit in an amount of 3mg or more, in particular in an amount of 3-6mg, as a contraceptive agent reducing the number of bleeding episodes by 5% or more, preferably 10% or more, in overweight women compared to non-overweight women subjected to the same contraceptive treatment.

Further, the experimental data disclosed herein show that the reduction in days to bleeding per treatment cycle tends to increase with the duration of the contraceptive therapy with the DRSP-POC formulation, achieving a reduction of 15% or more. As disclosed in the examples herein, the number of days of bleeding events per treatment cycle in overweight women is reduced by greater than 30% over the period of treatment cycles 2-9.

Still further, the present invention relates to the use of drospirenone as sole active ingredient contained in a daily active dosage unit in an amount of 3mg or more, especially in an amount of 3-6mg, as a contraceptive which reduces the number of bleeding episodes in obese women by 10% or more compared to non-obese women subjected to the same contraceptive treatment. As disclosed in the examples herein, the number of bleeding episodes in obese women is reduced by greater than 45% over the period of treatment cycles 2-9.

As further shown in the examples herein, the number of days of bleeding episodes in overweight or obese patients is no more than about 20%, 15%, 10%, 8%, or 5% in any treatment cycle following the initial treatment cycle.

Daily active dose of 1-drospirenone

In one aspect, the invention relates to the use of DRSP as sole contraceptive ingredient contained in a daily active dosage unit in an amount of at least 3mg as a contraceptive for overweight female patients, including overweight female patients as well as obese female patients.

In a particular embodiment, the present invention relates to the use of DRSP as sole contraceptive ingredient contained in a daily active dosage unit in an amount of at least 3mg for use as a contraceptive for obese female patients.

In certain embodiments, the present invention relates to DRSP as the sole contraceptive ingredient contained in a daily active dosage unit in an amount of at least 3mg for use as a BMI of 30kg/m²Or a female patient as above.

As used herein, "contraceptive component" refers to a component that prevents pregnancy when administered daily to a female patient in an effective amount over a period of 21-28 consecutive days. Contraceptive compositions can prevent pregnancy from occurring through a variety of biological effects. For example, pregnancy can be prevented by inhibiting ovulation, by thickening cervical mucus (which reduces sperm motility and penetration), and/or by preventing embryo implantation.

As used herein, the expression "the only contraceptive ingredient" is intended to mean that DRSP is the only ingredient that promotes contraception. In other words, the contraceptive component does not contain estrogen.

Drospirenone or DRSP, 6 β,7 β,15 β,16 β -dimethylene-3-oxo-17 a-pregn-4-ene-21, 17-carboxylactone and further identified as CAS registry number 67392-87-4, is a synthetic progestin with pharmacological profiles very close to that of natural progesterone.

The term "drospirenone" as used herein refers to drospirenone itself, drospirenone solvates and drospirenone derivatives or prodrugs.

DRSP may be prepared by well known methods described in the prior art, for example as described in US4129564, WO9806738, EP11746101 or WO 2006061309. The method described in WO2006061309 may be particularly suitable for the preparation of DRSP.

Needless to say, the method of preparing DRSP can be carried out to meet the requirements of Good Manufacturing Practice (GMP).

To ensure good bioavailability of DRSP, significant amounts of DRSP initially contained in the contraceptive composition must be released within a reasonable time frame.

Good bioavailability of DRSP can be achieved: the in vitro dissolution rate of DRSP of a composition comprising DRSP is such that at least 50% of the DRSP initially present in the composition dissolves in a period of 3 hours to 4 hours.

As used herein, an "active daily dosage unit" refers to a dosage unit that is capable of preventing pregnancy when administered daily to a female patient. In a preferred embodiment, the active daily dosage unit is capable of inhibiting ovulation.

As used herein, "female patient" refers to a female individual of childbearing age (i.e., from puberty to menopause). Female individuals of childbearing age also include perimenopausal women.

Body Mass Index (BMI) is a simple weight-height index commonly used to classify overweight and obesity in adults. Defined as the weight (kilograms) of a person divided by the square (kg/m) of his height (meters)²)。

The body weight standard status categories accepted by the World Health Organization (WHO) as being relevant to adult BMI ranges are as follows:

-less than 18.5kg/m²BMI of (a) indicates a too light condition;

–18.5kg/m²-24.9kg/m²BM ofI indicates normal weight status;

–25.0kg/m²-29.9kg/m²BMI of (a) indicates an overweight condition; and

–30.0kg/m²or higher BMI indicates an obese state.

As used herein, overweight female patients cover a BMI of 25.0kg/m²Or above, including overweight female patients and obese female patients.

As used herein, a female patient with overweight includes a BMI of 25.0kg/m²-29.9kg/m²For the female patient.

As used herein, an obese female patient or a female patient suffering from obesity encompasses a BMI of 30.0kg/m²Or a female patient of the above.

In certain embodiments, the BMI of the female patient is 25.0kg/m²Or more.

In certain preferred embodiments, the BMI of a female patient is 30.0kg/m²Or more.

In certain embodiments, the daily active dosage units are contained within a contraceptive kit, wherein the kit comprises one or more packaging units, wherein each packaging unit comprises 21-28 daily active dosage units, wherein:

a) the amount of DRSP in each daily active dosage unit is at least 3mg, estrogen-free, and

b) each daily active dosage unit comprises DRSP in a form such that:

(i) not more than 50% of the DRSP initially present in said daily active dosage unit dissolves in 30 minutes, and

(ii) at least 50% of the DRSP dissolves over a period of 3 hours to 4 hours,

when the daily active dose is subjected to an in vitro dissolution test according to USP XXIII paddle method, the percentage DRSP is relative to the amount of DRSP initially present in the daily active dose unit.

Daily active dosage units comprising DRSP are characterized by a slow in vitro dissolution rate of DRSP.

As used herein, "slow in vitro dissolution rate of DRSP" refers to the release of DRSP such that no more than 50% of the DRSP initially present in the daily active dosage unit dissolves in 30 minutes when the daily active dosage unit is subjected to a dissolution test.

As intended herein, no more than 50% of DRSP encompasses no more than 45%, 40%, 35%, 30%, 25%, 20%, 15%, 10% of the DRSP initially present in the daily active dosage unit.

In some embodiments, no more than 40% of the DRSP initially present in the daily active dosage unit is dissolved within 30 min.

As used herein, percentage DRSP is relative to the amount of DRSP initially present in the daily active dosage unit.

The in vitro dissolution rate of DRSP can be assessed by any of the well known methods described in the prior art.

The in vitro dissolution rate of DRSP is preferably assessed by the USP XXIII paddle method. Briefly, a tablet consisting of a contraceptive composition containing DRSP to be tested is placed in 900mL of water at 37 ℃ (± 0.5 ℃). Dissolution testing was performed using USP dissolution testing apparatus 2 with a stirring speed of 50 rpm.

In a preferred embodiment, the amount of DRSP in each daily active unit dose is at least 3mg of DRSP. DRSP of at least 3mg encompasses DRSP of at least 3.5mg, DRSP of at least 4mg, DRSP of at least 4.5mg, DRSP of at least 5mg or at least 5.5 mg.

In some embodiments, an active daily dosage unit consisting of a contraceptive composition as described above may contain a DRSP content of from about 3mg to about 6 mg. Daily dosages of about 3mg to about 6mg encompass daily dosages of 3.0mg, 3.5mg, 4.0mg, 4.5mg, 5.0mg, 5.5mg and 6 mg.

In certain embodiments, the DRSP content in each daily active unit dose is about 3.5mg to 4.5 mg.

As used herein, the term "about" preceding a "particular value" defines a range from "the particular value minus 10% of the particular value" to "the particular value plus 10% of the particular value". For example, "about 50" defines a range of 45-55.

In certain embodiments, one or more packaging units further comprise 1 to 7 daily dosage units of a pharmaceutically acceptable placebo.

Within the scope of the present invention, the term "placebo" is intended to mean a pharmacologically inert or harmless substance.

In certain embodiments, each packaging unit comprises 24 daily active dosage units.

In certain embodiments, each packaging unit comprises 4 daily placebo dosage units.

In certain embodiments, the DRSP is in a crystalline form.

In certain embodiments, the DRSP is in a non-micronized form.

As already mentioned, the crystalline and non-micronized forms of DRSP allow for slow in vitro dissolution rates of DRSP.

One way to obtain the DRSP-containing composition of the present invention is to use DRSP in a particulate form, e.g. non-micronized particulate form, having a suitable specific surface area. It has also been shown that the specific surface area is about 2000cm²G to about 8500cm²DRSP in particulate form per gram may be suitable to obtain the contraceptive compositions of the present invention. The specific surface area can be measured experimentally by the BET method (gas adsorption method).

In certain embodiments, the DRSP in particulate form has a specific surface area of about 2000cm²G to about 8500cm²/g。

Such specific surface area ranges include the following values: about 2000cm²/g、2500cm²/g、3000cm²/g、3500cm²/g、4000cm²/g、4500cm²/g、5000cm²/g、5500cm²/g、6000cm²/g、6100cm²/g、6200cm²/g、6300cm²/g、6400cm²/g、6500cm²/g、6600cm²/g、6700cm²/g、6800cm²/g、6900cm²/g、7000cm²/g、7500cm²G, 8000cm2/g and 8500cm 2/g.

With regard to the particle size distribution, DRSP particles with a diameter of more than 200 μm should be avoided so that the dissolution rate in vitro and thus the bioavailability in vivo is not very severely impaired, since such particles are poorly soluble.

In certain embodiments, d of DRSP₅₀Less than 70 μm.

In certain preferred embodiments, d of the DRSP particles₅₀Is 10-60 μm. D from about 10 μm to about 60 μm₅₀The ranges encompass d of 10 μm, 15 μm, 20 μm, 25 μm, 30 μm, 35 μm, 40 μm, 45 μm, 50 μm, 55 μm and 60 μm₅₀。

In some embodiments, the particle size distribution of DRSP present in the composition according to the invention is characterized by:

(i)d₉₀a particle size of less than about 100 μm, and/or

(ii)d₅₀A particle size of about 10 μm to about 60 μm, and/or

(iii)d₁₀The particle size is greater than about 3 μm.

In some other embodiments, d of the DRSP particles₅₀From about 10 μm to about 30 μm. In such an embodiment, the particle size distribution of the DRSP present in the composition according to the invention is characterized by at least one of the following features:

(i)d₉₀the particle size is less than about 100 μm,

(ii)d₅₀a particle size of from about 10 μm to about 30 μm, and

(iii)d₁₀the particle size is greater than about 3 μm.

As used herein, "d" is₉₀Particle size "means a particle size distribution such that at least 90% of the particles have a particle size diameter less than a specified value.

As used herein, "d" is₅₀Particle size "means a particle size distribution such that at least 50% of the particles have a particle size diameter less than a specified value.

As used herein, "d" is₁₀Particle size "means a particle size distribution such that at least 10% of the particles have a particle size diameter less than a specified value.

d₉₀Particle sizes less than about 100 μm include d₉₀Particle size less than about 90 μm, 80 μm, 70 μm, 60 μm, 55 μm, 50 μm, 45 μm, 40 μm, 38 μm, 36 μm, 34 μm、32μm、30μm、28μm、26μm、24μm、22μm、20μm。

d₅₀Particle size values of about 10 μm to about 30 μm include values of about 10 μm, 11 μm, 12 μm, 13 μm, 14 μm, 15 μm, 16 μm, 18 μm, 19 μm, 20 μm, 21 μm, 22 μm, 23 μm, 24 μm, 25 μm, 26 μm, 27 μm, 28 μm, 29 μm, 30 μm.

d₁₀Particle size values greater than about 3 μm including d₁₀The particle size is greater than about 3 μm, 3.5 μm, 4.5 μm, 5 μm, 6 μm, 7 μm, 8 μm, 9 μm, 10 μm, 11 μm, 12 μm.

Needless to say, d₁₀Particle size value less than d₅₀Particle size value, d₅₀Particle size value less than d₉₀Particle size value.

DRSP particle size distribution, especially d₉₀、d₁₀And d₅₀The values can be determined by methods well known in the art, such as sieve analysis, laser diffraction, light analysis or optical counting. The laser diffraction method is particularly preferable. The particle size distribution can be determined by laser diffraction in a wet dispersion. The dispersant is preferably water.

In some embodiments, the pharmaceutical compositions of the present invention comprise DRSP in particulate form having a particle size distribution with a combination of two characteristics selected from:

(i)d₉₀the particle size is less than about 100 μm,

(ii)d₅₀a particle size of from about 10 μm to about 30 μm, and

(iii)d₁₀the particle size is greater than about 3 μm.

In other words, the DRSP particle size distribution exhibits a combination of characteristics selected from characteristics (i) and (ii), characteristics (i) and (iii), and characteristics (ii) and (iii).

In some embodiments, the pharmaceutical compositions of the present invention comprise a non-micronized form of DRSP having a particle size distribution characterized by:

(i)d₉₀the particle size is less than about 100 μm,

(ii)d₅₀a particle size of from about 10 μm to about 30 μm, and

(iii)d₁₀the particle size is greater than about 3 μm.

In a preferred embodiment, the DRSP particle size distribution is further characterized by d₉₀The particle size value is less than 50 μm and the particle size of no particle is greater than 80 μm.

In some embodiments, the contraceptive compositions of the present invention comprise DRSP in particulate form, d thereof₉₀Particle size of about 20 μm to about 40 μm, d₅₀A particle size of from about 10 μm to about 30 μm and a d10 of from about 3 μm to about 9 μm, and wherein no particle has a particle size of greater than 80 μm, more preferably no particle has a particle size of greater than 60 μm.

In some other embodiments, the contraceptive compositions of the present invention comprise drospirenone in particulate form which:

(i)d₉₀a particle size of about 30 μm to about 40 μm,

(ii)d₅₀a particle size of from about 15 μm to about 25 μm, and

(iii)d₁₀from about 5 μm to about 9 μm, and wherein no particle has a particle size greater than 80 μm, more preferably no particle has a particle size greater than 60 μm.

In some other embodiments, the contraceptive compositions of the present invention have a specific surface area of about 2000cm²Per gram to about 8000cm²G and d₅₀DRSP in the form of particles having a particle size of 10 μm to 60 μm.

To obtain DRSP in the form of particles having a specific surface area and/or particle size distribution as described above, a person skilled in the art can use well known methods of the prior art, such as a milling process, optionally in combination with a sieving process.

For example, DRSP obtained by any of the syntheses described in the prior art may be subjected to a ball mill or hammer mill step, optionally followed by a vibratory screening step. Subsequent vibratory screening steps can remove the finest and largest particles of DRSP that can impair the pharmacokinetic and in vitro dissolution profiles of DRSP.

The parameters of the milling and sieving steps can be adjusted by one skilled in the art by routine experimentation to obtain the appropriate particulate form of DRSP. Suitable mills that may be used include hydraulic mills, ball or rod mills, hammer mills, cutting machines and vibratory pelletizers.

Suitable DRSP in particulate form can also be prepared by crystallization or precipitation methods, optionally in combination with a sieving step, to fully control the DRSP particle size. The precipitation method may comprise the steps of: (i) dissolving DRSP in a water miscible solvent, and then (ii) dispersing the resulting solution in cold water with stirring to induce precipitation of DRSP. The DRSP particles can then be recovered by filtration.

The water-miscible solvent may be a solvent which is generally used in crystallization or precipitation methods, such as methanol, ethanol, isopropanol, dimethylformamide, tetrahydrofuran, dioxane or dimethyl sulfoxide, dimethylacetamide or acetone.

This process enables DRSP to be obtained in substantially crystalline form.

The parameters of the precipitation method to be used can be determined by the skilled person by routine experimentation to obtain the appropriate form of DRSP.

The parameters of the precipitation process (e.g. the amount of solvent, the amount of water and optionally the amount of surfactant to be used) can be adjusted by the skilled person by routine experimentation.

2-methods of contraception

The present invention also relates to methods comprising administering to a overweight female patient a pharmaceutical composition comprising DRSP in an amount of at least 3mg, wherein the pharmaceutical composition allows a 28-day daily dosing regimen, and wherein the initial administration of the DRSP after establishing its contraceptive effect in the patient, the patient can skip up to 4 doses within a 28-day daily dosing regimen cycle.

In some embodiments, the invention also relates to methods comprising administering to an obese female patient a pharmaceutical composition comprising DRSP in an amount of at least 3mg, wherein said pharmaceutical composition allows a 28 day daily dosing regimen, and wherein the initial administration of said DRSP after establishing its contraceptive effect in the patient, said patient can skip up to 4 doses within a 28 day daily dosing regimen cycle.

In some embodiments, the invention further relates to a method comprising administering to a BMI of 30kg/m²Or higher, in a female patient, administering a pharmaceutical composition comprising DRSP in an amount of at least 3mg,wherein the pharmaceutical composition allows a 28 day daily dosing regimen, and wherein after initial administration of the DRSP establishes its contraceptive effect in a patient, the patient may skip up to 4 doses over a 28 day daily dosing regimen cycle.

In some embodiments of the above methods, the female patient has a BMI of 25kg/m²Or higher.

In some embodiments of the above methods, the female patient is obese.

In certain embodiments, the female patient has a BMI of 30kg/m²Or higher.

As used herein, a "daily dosing regimen" refers to a method of contraception in a female patient suffering from obesity comprising the step of administering to said female patient an active daily dosage unit consisting of a pharmaceutical composition as substantially described herein over a period of consecutive days over a 28-day period (i.e. a period of time corresponding to the average length of a menstrual cycle).

As used herein, an "active daily dosage unit" refers to a dosage unit that is capable of preventing pregnancy when administered daily to a female patient over a period of 28 consecutive days.

After the DRSP has established its contraceptive effect, the method may include a second phase of the non-contraceptive period (i.e., the period in which no contraceptive ingredient is administered to the female patient). During the second phase, daily placebo dosage units may be administered to the female patient. In some other cases, the pill is not administered to the female patient.

By "daily placebo dosage unit" is understood that the ingredients contained in the dosage unit are pharmaceutically inert or non-hazardous. In other words, the daily placebo dosage unit does not comprise any contraceptive ingredient as defined herein.

This second phase may allow regular menstrual bleeding to occur, thus simulating the natural menstrual cycle.

Furthermore, it is believed that the second phase results in the secretion of endogenous estradiol, which may have some benefit to the bone metabolism of female patients.

In certain embodiments, the pharmaceutical composition further allows the patient to skip up to two consecutive days of the DRSP during the 28-day daily dosing regimen, provided that the skipped dose of DRSP is taken within about 24 hours of the skipped up to two consecutive days.

In certain embodiments, the skipped up to 4 doses are on non-consecutive days.

In certain embodiments, the skipped up to 4 doses are on consecutive days.

In a general aspect, it is preferred to use non-micronized and crystalline forms of DRSP to prepare the pharmaceutical compositions of the present invention.

The daily dosing regimen of DRSP to be administered to an obese female patient may also be adjusted depending on individual factors such as the female patient's age, weight, overall health, and diet. The daily dosing regimen may also vary based on drug interactions that may occur. The daily dosing regimen may also vary based on other biological effects than those expected by administration of DRSP in the prevention of pregnancy.

The daily dosing regimen of DRSP to be administered daily to a female patient may be lower or higher than the dosages mentioned previously. For example, perimenopausal female patients may require higher or lower daily doses of DRSP to improve their overall condition, e.g., to improve their menstrual cycle regularity.

Adjustment of the daily dosing regimen can be routinely determined by the physician.

In a preferred embodiment, the pharmaceutical composition of the invention further comprises one or more pharmaceutically acceptable excipients.

The pharmaceutical compositions of The present invention may be formulated according to standard methods, such as those described in Remington: The Science and Practice of Pharmacy (Lippincott Williams & Wilkins; twin first Edition, 2005).

Pharmaceutically acceptable Excipients that may be used in formulating the contraceptive compositions of the present invention are described, inter alia, in Handbook of Pharmaceuticals Excipients, American Pharmaceutical Association (Pharmaceutical Press; 6)^thRevised edition, 2009).

Examples of suitable excipients include, but are not limited to, fillers, carriers, diluents, binders, anti-caking agents, plasticizers, disintegrants, lubricants, flavoring agents, buffering agents, stabilizers, colorants, dyes, antioxidants, anti-adherents, softeners, preservatives, and glidants.

In some embodiments, the contraceptive compositions of the present invention comprise one or more excipients selected from the group consisting of binders, fillers, glidants, and lubricants.

Examples of fillers include, but are not limited to, lactose anhydrous, microcrystalline cellulose, starch, pregelatinized starch, modified starch, dibasic calcium phosphate dihydrate, calcium sulfate trihydrate, calcium sulfate dihydrate, calcium carbonate, lactose, dextran, sucrose, mannitol, and sorbitol, and combinations thereof.

Examples of lubricants include, but are not limited to, magnesium stearate, calcium stearate, zinc stearate, talc, propylene glycol, PEG, stearic acid, vegetable oils, sodium benzoate, sodium lauryl sulfate, magnesium lauryl sulfate, mineral oil, polyoxyethylene monostearate, and combinations thereof.

Examples of binders include, but are not limited to, starches, such as potato starch, wheat starch, corn starch; gums, such as tragacanth, acacia and gelatin; microcrystalline cellulose, hydroxypropyl cellulose, hydroxyethyl cellulose, and hydroxypropyl methyl cellulose; polyvinylpyrrolidone and combinations thereof.

Examples of glidants include silicon dioxide, magnesium trisilicate, powdered cellulose, starch, talc and tribasic calcium phosphate.

In a preferred embodiment, the pharmaceutical composition according to the invention does not comprise a significant content of surfactant. Significant levels of surfactant can impair the in vitro dissolution profile of DRSP by increasing its initial dissolution rate. Surfactants include non-ionic surfactants such as polyoxyethylene sorbitan fatty acid esters and ionic surfactants such as sodium lauryl sulfate.

Needless to say, the DRSP to be used may have a specific surface area and/or d as fully described in the present specification₉₀、d₁₀And d₅₀Particle size in the form of granules.

The contraceptive composition may optionally include additional excipients that may comprise from about 0.1% to 10% by weight.

Contraceptive compositions according to the invention may be formulated in galenic form suitable for oral administration. Such forms include, but are not limited to, tablets, troches, granules, pills, capsules, powders, and suspensions.

In a preferred embodiment, the contraceptive composition is formulated for oral administration in solid form, such as tablets, capsules, granules, lozenges and pills.

Such solid forms are particularly suitable for use as daily active dosage units in a contraceptive kit according to the invention.

When the pharmaceutical composition is formulated into a solid form, such as a tablet or pill, the solid form may be conveniently coated with a suitable film forming agent as follows: e.g., hydroxypropylmethylcellulose, hydroxypropylcellulose or ethylcellulose, to which suitable excipients, e.g., softeners such as glycerol, propylene glycol, diethyl phthalate or glyceryl triacetate; fillers such as sucrose, sorbitol, xylitol, glucose or lactose; or a colorant such as titanium hydroxide or the like.

Pharmaceutical compositions in the form of tablets, pills or granules can be prepared by conventional methods such as direct compression, dry granulation and wet granulation.

In some embodiments, the solid form is obtained by direct compression.

It is a further object of the present invention to provide a method of preparing a contraceptive composition as described herein, comprising the steps consisting of:

(i) providing DRSP in particulate form substantially as herein before described;

(ii) providing one or more pharmaceutically acceptable excipients; and

(iii) (iii) mixing the DRSP provided in step (i) with one or more excipients provided in step (ii).

As fully described above, applicants provide technical guidelines to obtain compositions comprising DRSP in a form such that:

(i) not more than 50% of the DRSP initially present in the composition dissolves in 30 minutes, and

(ii) at least 50% of the DRSP dissolves over a period of 3 hours to 4 hours,

when the composition is subjected to an in vitro dissolution test, the percentage DRSP is relative to the amount of DRSP initially present in the composition.

Compositions comprising DRSP having such in vitro dissolution profiles or in vivo pharmacokinetic profiles as fully described above may be achieved in a variety of other ways.

By routine experimentation and based on its general knowledge, one skilled in the art can vary (i) the particle size distribution of DRSP and (ii) the amount and nature of excipients to obtain other alternative compositions that exhibit the in vitro dissolution profile or in vivo pharmacokinetic profile described herein.

For example, one skilled in the art would expect a composition comprising (i) micronized DRSP and (ii) a sustained release agent to reduce the rate of dissolution of the DRSP.

One skilled in the art would also contemplate combining (i) large particles of DRSP with (ii) surfactants and/or wetting agents to ensure dissolution of the DRSP.

In certain embodiments, the pharmaceutical composition comprises a contraceptive kit comprising one or more packaging units, wherein each packaging unit comprises 21-28 daily active dosage units, wherein:

a) the drospirenone content of each daily active dosage unit is at least 3mg, free of estrogen, and

b) each daily active dosage unit comprises drospirenone in a form such that:

(i) not more than 50% of the drospirenone originally present in said daily active dosage unit is dissolved within 30 minutes, and

(ii) at least 50% of said drospirenone is dissolved in a time ranging from 3 hours to 4 hours.

When the daily active dose is subjected to an in vitro dissolution test according to USP XXIII paddle method, the percentage drospirenone is relative to the amount of drospirenone originally present in said daily active dose unit.

The contraceptive kit comprises one or more packaging units.

The one or more packaging units include, but are not limited to, 1 packaging unit, 2 packaging units, 3 packaging units, 4 packaging units, 5 packaging units, and 6 packaging units.

Each packaging unit contains 21-28 daily active dose units. As fully set forth above, each daily active dosage unit consists of a contraceptive composition of the present invention.

As fully described above, the daily active dosage unit preferably does not contain any estrogen or estrogen derivative such as ethinyl estradiol, ethinyl estradiol methyl ether or 8-prenylnaringenin. In other words, DRSP is preferably present in daily active dosage units that do not contain estrogen.

In a more preferred embodiment, DRSP is the only contraceptive ingredient contained in the daily active dosage unit.

Each packaging unit optionally contains 1-7 daily dosage units of a pharmaceutically acceptable placebo.

In some embodiments, the contraceptive kit is characterized in that each packaging unit comprises 28 daily dosage units and does not contain a pharmaceutically acceptable placebo daily dosage unit. Such contraceptive kits are particularly suitable for performing the contraceptive methods of the present invention, which consist in "continuous" administration of DRSP without a period of non-avoidance.

In other embodiments, each packaging unit comprises

21 to 27 daily dosage units of a contraceptive composition substantially as described herein, and

-optionally, 1 to 7 pharmaceutically acceptable placebo daily dosage units.

Such a contraceptive kit is particularly suitable for carrying out the contraceptive method of the invention, which comprises:

-a first phase wherein an active daily dosage unit of the invention containing no estrogen is administered to an obese female patient over a period of 21 to 27 consecutive days, followed by;

-a second phase wherein the female patient is not administered a contraceptive composition for a period of 1 to 7 consecutive days.

In some other embodiments, each packaging unit of the kit comprises 4 daily dosage units comprising an effective amount of 24 daily dosage units of the contraceptive composition described herein and optionally a pharmaceutically acceptable placebo.

The above-described packaging unit may have a conventional form commonly used for oral contraceptives.

For example, the packaging unit may be a conventional blister pack comprising an appropriate number of dosage units in a blister pack sealed with a cardboard, paperboard, foil or plastic backing and enclosed in a suitable cover. Each blister container may be conveniently numbered or marked to promote compliance.

The packaging units may comprise daily dosage units in the order in which they are to be taken, i.e. starting with the first of at least 21 dosage units comprising the DRSP composition, optionally followed by 7 or less empty blisters, or 7 or less dosage units comprising a pharmaceutically acceptable placebo.

The kits of the invention may comprise other suitable components, such as instructions for use.

The following examples are illustrative and are not intended to limit the scope of the invention as claimed.

Detailed Description

Example 1 LF111(DRSP) treatment reduces the days of bleeding and/or spotting

1/target

The following study CF111/302 represents a critical, multicenter, double-blind, double-simulated randomized trial of contraceptive efficacy, tolerability, and safety of LF111(DRSP) over 9 cycles of 28-day treatment (24 active test product tablets followed by 4-day placebo tablets).

The first objective was to demonstrate contraceptive efficacy of LF111, and the second objective was to demonstrate safety and tolerability of LF111, especially in terms of bleeding patterns.

2/materials and methods

a) Test product, dosage and mode of administration

LF111 film coated tablets (test product: 24 tablets containing 4mg DRSP followed by 4 placebo tablets LeLo Lo n Farma) were administered orally during this trial. LF111 tablets have the following formulation:

¹crystalline and non-micronized drospirenone, prepared according to a process similar to that described in WO 2006/061309;

b) design of experiments

The prospective, multicenter, randomized, double-blind, double-simulated trial was performed in 857 women with no uncontrolled current disease, at risk of pregnancy, age 18-45, systolic pressure <140mmHg, diastolic pressure <90mmHg, followed in approximately 73 centers in austria, czech, germany, hungary, polish, roman, swania, silovak and spain.

After visit 1a (screening; V1a) provided informed consent and after visit 1b received study medication, subjects will participate in visits 2-4 on days 24 ± 2 of the first, third and sixth cycles and visit 5 on days 29+2 of the ninth cycle (V5). Follow-up visits (visit 6; V6) will be made 7-10 days after the last LF111 intake.

The total duration of the planned trial was set to 16 months with a recruitment process of up to 6 months, a contraceptive therapy itself of up to 9 months and a follow-up procedure of 10 days. The duration of contraceptive therapy for an individual woman was 9x28 days.

c) Exclusion criteria

A pregnant subject; a mammalian subject; subjects known or suspected to be unable to follow the study protocol and to use IMP (pharmaceutical product of study); abnormal findings on pelvic, breast or vaginal ultrasonography that hamper participation in the trial; unexplained menopause-free, known polycystic ovarian syndrome; subjects with ASC-US or more severe findings on Pap smears; contraindications or hypersensitivity to the active ingredient (drospirenone) or excipients (cellulose, lactose, silicon dioxide, magnesium stearate, corn starch, polyethylene glycol, polyvinylpyrrolidone, polyvinyl alcohol, aquarius BT16035 cottage green, talc, titanium dioxide; colloidal anhydrous silicon dioxide, all-rac-alpha-tocopherol, lactose, monohydrate, corn starch, povidone, stearic acid, hypromellose, polyethylene glycol 400) of IMP are known; severe cardiovascular disease, liver or kidney disease, diabetes with vascular involvement, uncontrolled thyroid disease or recent venous thrombosis or embolism; undiagnosed vaginal bleeding; a known or suspected sex hormone sensitive malignancy; the presence or history of severe liver disease, as long as the functional value of the liver does not return to normal; evidence or medical history of abuse of alcohol, drugs or drugs (within the last 12 months); a known history of bleeding disorders or unexplained bleeding or bruising within the last 12 months prior to V1 a; prohibited previous drugs/contraceptives (injectable contraceptive hormone method in the last 6 months before V1a, progestogen releasing IUD (intrauterine device) or contraceptive implant in the last 2 months before V1a, antiretroviral therapy in the last 6 months before V1a, microsomal enzyme induced drug in the last 28 days before IMP start); dependence on combination medication prohibited as follows: estrogens, progestogens, activated charcoal, microsomal enzyme-inducing drugs, anticonvulsants [ e.g., hydantoin, phenytoin, carbamazepine, oxcarbazepine, topiramate, febuxostat, primidone ], barbiturates, antibiotics [ e.g., rifabutin or rifampin ], ritonavir, nelfinavir, atorvastatin, bosentan, griseofulvin, phenylbutazone, st. john wort [ hypericum ], drugs that can increase serum potassium [ ACE inhibitors, angiotensin-H receptor antagonists, potassium sparing diuretics, potassium supplements, heparin, aldosterone antagonists, and NSAIDs ]; planning a surgical procedure within the expected time of participation in the trial, requiring the abandonment of oral contraceptives; regular concurrent use of barrier contraceptive methods, spermicides, IUDs or other contraceptive measures (except for occasional use due to risk of infection); signs or history of neurologic personality, psychosis, or suicide; participating in or previously participating in a test of another study drug or device in parallel with the test or less than 90 days prior to entering the test; an employee of the researcher or testing center, or an employee or family member of the researcher; any situation in which the test according to this protocol is carried out may be jeopardized according to the opinion of the researcher.

d) Evaluation criteria

d.1) efficacy

-essentially: overall pel index (overall PI);

-a secondary: PI of the method loser; corrected PI for future contraception; the rate of pregnancy.

d.2) safety/tolerability

Proportion of subjects with episodic/spotting bleeding in cycles 2-6; the proportion of subjects with episodic/spotting bleeding that accumulated in each of cycles 2-9 and cycles 2-6 and 2-9; days of bleeding/spotting during cycle 2-4; days of bleeding/spotting during cycle 7-9; days of bleeding/spotting during cycle 2-9; number of bleeding/spotting episodes during cycle 2-4; number of bleeding/spotting episodes during cycles 7-9; number of bleeding/spotting episodes during cycles 2-9; proportion of subjects without menstruation; body weight change from baseline (V1 a); systolic and diastolic blood pressure changes from baseline (V1 a); adverse Events (AE); a pulse rate; electrocardiograms (ECGs) of a subset of subjects; clinical laboratory parameters; specific clinical laboratory parameters (hemostatic variables, carbohydrate metabolism and bone metabolism) of a subgroup of subjects.

d.3) statistical methods

d.3.1) efficacy parameters

The total analysis set (FAS) and each protocol set (PPS), an analysis of the main efficacy variables defined as overall PI will be performed. The primary efficacy evaluation will be based on FAS. Given that the pregnancy events had a poisson distribution, bilateral 95% Confidence Intervals (CI) for the overall PI would be calculated. The secondary efficacy analysis will be based on FAS. Two-sided 95% CI of method loser PI will be calculated. The cloner-Pearson 95% confidence interval for pregnancy rates will be calculated. The cumulative pregnancy rate will be calculated using a Kaplan Meier estimator. For corrected PI for backup contraception, bilateral 95% CI will be calculated.

d.3.2) safety and tolerability parameters

The analysis of the parameters blood pressure, body weight and bleeding pattern will be based on FAS. The analysis of the security endpoints will be performed using only the security analysis set. All Adverse Events (AEs) and Treatment Emergency Adverse Events (TEAEs) will be summarized by calculating the number and percentage of subjects with AEs by preference and systemic organ classification. In addition, TEAE will be summarized by severity and relationship to treatment. The number and percentage of TEAEs that led to study termination will be provided. Laboratory parameters, pulse rate and abnormal ECG results (e.g., QT prolongation) will be summarized by calculating the absolute values and summary statistics of the changes from V1a (specific laboratory parameters and ECG: V1b) to V3, V4 and V5. A shift table will be provided to illustrate the variation from the normal range of the laboratory between V1a and V5 (or EDV). The number and percentage of subjects with values outside the limits of clinical significance will be summarized.

2/result

As shown in Table 1 below, the DRSP treatment was overweight (BMI 25 kg/m) compared to non-overweight women on the same contraceptive treatment²Or higher) women have a significantly reduced number of days to bleed and/or spotting.

Table 1: the overweight women treated the treatment cycle time for punctate bleeding and/or the number of days of bleeding compared to the non-overweight women treated with DRSP.

N: number of subjects in the test group in a particular BMI group

n: number of subjects with available data

SD: standard deviation of

As shown in table 2 below, the days of bleeding and/or spotting in DRSP treated obese women were significantly reduced compared to non-obese women on the same contraceptive treatment.

Table 2: the obese women group treated the punctate bleeding and/or the number of days of bleeding over the cycle time compared to the non-obese women group treated with DRSP.

N: number of subjects in the test group in a particular BMI group

n: number of subjects with available data

SD: standard deviation of

Example 2-correlation between BMI and DRSP-based treatment on the one hand and weight changes and Heart Rate changes on the other hand

1/method

In example 2, the LF111 formulation described in example 1 was used.

The CF111/301 clinical trial protocol included 713 healthy sexually active women willing to use an oral contraceptive, recruited in approximately 41 centers located in 5 countries (hungarian, polish, czech, germany and romania).

After visit 1a (screening) signed an informed consent and after visit 1b received study medication, eligible subjects will participate in visits 2 through 6 on days 24 ± 2 of the first, third, sixth, ninth and thirteenth cycles. Follow-up was performed 10 to 28 days after visit 6 (visit 7). At least 515 subjects completed a respective 13 cycles of study.

The data set has information on demographic and clinical parameters, gynecological and medical history data, laboratory and vital signs assessment, prior/concomitant medication/contraceptive device related data.

Statistical p-values were calculated using the Fisher's exact test, and p-values were found to be significant at the threshold p ≦ 0.05.

2/result

For obesity (BMI is more than or equal to 30 kg/m)²) Women, a decreasing trend in their body weight and heart rate was observed from visit 1 (measured at baseline) to visit 6 (measured at the end of the study).

BMI group (BMI)<30 and BMI more than or equal to 30kg/m²) The distribution of the change in body weight and heart rate from visit 1 to 6 in the women is shown in figures 1 and 2, respectively.

The linear model analysis showed that the effect of the BMI group on changes in body weight (F-statistic: 14.49 at 1, DF 668, p-value: 0.0001541) and heart rate (F-statistic: 4.947 at 1, DF 666, p-value: 0.02647) was statistically significant from visit 1 to 6.

Example 3 reduction of side effects of DRSP-based treatment in obese women

1/method

The CF111/1SS clinical trial protocol included 1571 (1500+71) healthy sexually active women willing to use oral contraceptives recruited to approximately 114 centers located in austria, czech, germany, hungary, poland, romania, silovax and spain. Separate study of non-obese female group (BMI)<30kg/m²) And obese women (BMI greater than or equal to 30 kg/m)²)。

2/result

Table 3 describes the quantitative results of TEAE (treatment of emergency adverse events) in the group of women contraceptive with a composition comprising drospirenone ("LF 111") according to the invention. Study of non-obese women (BMI) separately<30kg/m²) And obese women (BMI greater than or equal to 30 kg/m)²)。

The results disclosed in table 3 indicate that the percentage of TEAE was similar in women using the DRSP-POC formulation, regardless of whether these women were obese or non-obese.

Therefore, the results indicate that obese women should have a high adherence (observence) rate to the drospirenone containing formulation according to the invention.

Table 3: TEAE incidence in BMI subgroup individuals treated with LF111

N: number of subjects in a particular treatment group

n: number of subjects with adverse events

Percent: percentage based on N

[a] TEAE: treatment of an emergency adverse event. TEAE is defined as an AE that begins at or after the first administration of IMP and includes those events that begin before the first administration of IMP but worsen after the first intake. Adverse events starting after the last IMP administration but within the follow-up time after the last IMP would be considered treatment urgent.

Heart rate is understood as the number of beats per minute of a person at rest (e.g. not in motion). Preferably, the heart rate may be measured after the patient has laid down for at least 5 minutes, preferably at least 10 minutes and most preferably at least 15 minutes. Alternatively, heart rate may be measured before waking up in the morning and getting out of bed. Heart rate is an important health indicator.

While a normal heart rate for an adult may be about 60 to about 100 beats per minute, a lower heart rate indicates more effective cardiac function and cardiovascular health. Although overweight and obese women are generally observed to have a higher heart rate than normal weight women, it has also been found that a faster heart rate is a warning sign of increased cardiovascular problems and also serves as a predictor of obesity later in life. It has been found that women with a higher heart rate are prone to obesity and diabetes. Shigetoh, et al, am.j.hypertension, vol.22, number 2, pp.151-155, feb.2009. It is believed that higher heart rates are associated with metabolic syndrome, diabetes, blood clot formation that can cause stroke or heart attack, heart failure, syncope and even sudden death.

Therefore, a reduced heart rate is highly desirable, especially for overweight or obese women, as reducing heart rate can result in a reduced risk of developing various detrimental health conditions. It is believed that lowering the heart rate at least 5 times per minute, at least 10 times per minute, and at least 15 times per minute significantly reduces this risk factor.

Example 4-comparison of bleeding or spotting episodes observed in obese women using DRSP-based and desogestrel-based treatments according to the present invention

1/purpose

The following study CF111/302 represents a critical, multicenter, double-blind, double-simulated randomized trial of contraceptive efficacy, tolerability, and safety of LF111(DRSP) compared to desogestrel 0.075mg (cerazette) over 9 cycles.

The first objective was to demonstrate contraceptive efficacy of LF111, and the second objective was to demonstrate safety and tolerability of LF111 compared to desogestrel 0.075mg, especially in terms of bleeding patterns.

2/materials and methods

a) Test and reference products, dosages and modes of administration

Two tablets were administered orally during the trial:

LF111 film coated tablets (test product: 24 tablets containing 4mg DRSP, followed by 4 placebo tablets LeLo Lo n Farma). The formulation of LF111 tablets is disclosed in example 1 (see section 2, subsection a)).

Desogestrel 0.075mg film-coated tablet (reference: 28 active tablets, NV Organon).

b) Design of experiments

Prospective, multicentric, randomized, double-blind, double-simulated experiments were performed in 1200 women with an approximately 88-center follow-up in austria, czech, germany, hungary, polish, roman, romania, silovak and spain (LF111 for position 857 and desogestrel 0.075mg for position 343; randomized ratio 5:2) without uncontrolled current disease, at risk of pregnancy, age 18-45 years, systolic pressure <140mmHg, diastolic pressure <90 mmHg.

After visit 1a (screening; V1a) provided informed consent and after visit 1b received study medication, subjects will participate in visits 2 through 4 on days 24 ± 2 of the first, third and sixth cycles and visit 5 on days 29+2 of the ninth cycle (V5). Follow-up visits (visit 6; V6) will be made 7-10 days after the last LF111 intake.

The total duration of the planned trial was set to 16 months with a recruitment process of up to 6 months, a contraceptive therapy itself of up to 9 months and a follow-up procedure of 10 days. The duration of contraceptive therapy for each woman was 9x28 days.

c) Exclusion criteria

See the relevant section above for example 1.

d) Evaluation criteria

See the relevant section above for example 1.

3/result

Tables 4 and 5 show that DRSP-based therapy (LF111) and desogestrel-based (as available) according to the inventionPills commercially available) of the treatment of obese women.

Table 4: obese female subjects (BMI ≧ 30 kg/m) relative to treatment received²) Bleeding or spotting observed in

Table 5: normal or overweight female subject (BMI) relative to the treatment received<30kg/m²) Bleeding or spotting observed in

It was observed that obese female subjects administered the drospirenone-based contraceptive therapy disclosed herein experienced significantly fewer bleeding or spotting episodes at all cycle phases analyzed as compared to obese female subjects receiving treatment based on a contraceptive composition comprising desogestrel.

In contrast, BMI is less than 30kg/m²(ii) female individuals (including individuals with normal weight or individuals with excess weight) experience the same or equal bleeding or spotting event regardless of which contraceptive therapy is being administered.