阅读说明：本技术 一种海参肽化活性钙制品及其制备方法 (Sea cucumber peptidation active calcium product and preparation method thereof ) 是由 高玉志 于 2019-10-23 设计创作，主要内容包括：本发明涉及一种海参肽化活性钙制品及其制备方法,属于海洋生物制品领域,其特征是以氯化钙为原料,经海参肽化处理、透析、干燥、包装工序,制得稳定性好、吸收率高的海参肽化活性钙补充制剂；该工艺不需要昂贵复杂的设备,生产成本低,易于实现产业化生产,是一种绿色加工技术；本发明生产的海参肽化活性钙,颗粒均匀,稳定性好,吸收率高,可改善骨质疏松,增强骨密度,是一种极具市场推广前景的活性钙补充制剂。(The invention relates to a sea cucumber peptidation active calcium product and a preparation method thereof, belonging to the field of marine biological products, and being characterized in that calcium chloride is used as a raw material, and a sea cucumber peptidation active calcium supplement preparation with good stability and high absorption rate is prepared through sea cucumber peptidation treatment, dialysis, drying and packaging; the process does not need expensive and complicated equipment, has low production cost, is easy to realize industrialized production, and is a green processing technology; the sea cucumber peptide active calcium produced by the invention has uniform particles, good stability and high absorption rate, can improve osteoporosis and enhance bone mineral density, and is an active calcium supplement preparation with great market popularization prospect.)

1. A preparation method of a sea cucumber peptidation active calcium product is characterized by comprising the following steps:

1) preparation of sea cucumber bioactive peptide: adding 0.01-0.05% of trypsin into each gram of sea cucumber for enzymolysis, preparing sea cucumber enzymolysis liquid under the conditions that the enzymolysis temperature is 50 ℃, the enzymolysis time is 6 hours and the pH value is 7.0, and obtaining the sea cucumber active peptide by Sephadex G-25 gel column chromatography and spray drying of the enzymolysis liquid;

2) and (3) peptidation treatment: carrying out composite reaction on a calcium chloride solution with the mass ratio of 5% and a sea cucumber bioactive peptide solution with the mass ratio of 10% to form a peptide bioactive calcium solution, wherein the peptide condition is as follows: the mass ratio of the peptide to the calcium chloride is 3-5: 1, the reaction temperature is 37-50 ℃, and the reaction time is 30-60 min;

3) and (3) dialysis: removing free calcium ions and chloride ions subjected to peptidation in the step 2) by dialysis treatment;

4) and (3) drying: spray drying the solution dialyzed in the step 3), and adding a mannitol protective agent with the mass ratio of 5% before drying;

5) packaging: packaging the dried peptide activated calcium in the step 4) to obtain the sea cucumber peptide activated calcium product.

2. The method for preparing a sea cucumber peptidation activated calcium product according to claim 1, wherein the peptidation condition is that the mass ratio of the peptide to the calcium chloride is 5: 1, the reaction temperature is 50 ℃, and the reaction time is 30 min.

3. The method for preparing sea cucumber peptidation activated calcium product according to claim 1, wherein the molecular weight of the sea cucumber activated peptide is less than 1000 Da.

4. A sea cucumber peptidation activated calcium product, which is prepared by the preparation method of the sea cucumber peptidation activated calcium product of any one of claims 1 to 3.

Technical Field

The invention belongs to the field of marine medicines and biological products, and particularly relates to a sea cucumber peptidation active calcium product and a preparation method thereof.

Background

Calcium is one of mineral elements essential to human body, and plays an important role in various vital activities such as bone composition, muscle contraction, cell phagocytosis and enzyme activation. Calcium in human body mainly comes from food, and calcium deficiency has become one of global nutrition problems due to low absorption and utilization rate of calcium. The national diet survey shows that the intake of calcium in the diet is less than half of 800mg (49.2%) required daily supply, the city is slightly high (53.3%), the rural area is low (47.1%), and the calcium deficiency of children, pregnant women and old people is more obvious.

At present, the problem of aging in China is very prominent, and according to statistics, in 2001, the proportion of the population of the aged 65 years old and older in China reaches 7.1 percent, and the aged people formally enter the aging society according to the standard of the United nations; the population number of China over 65 years old in 2014 is up to 13755 million people, accounts for 10.1 percent of the whole country, and still continuously increases. According to the statistics and aging of the united nations, the worldwide trend that the age of the 21 st century is irreversible is achieved, the aging speed of the Chinese society is faster than the general population growth speed of the whole country, and therefore the fields of increasing bone density and effectively treating osteoporosis are widely concerned by people.

Calcium preparations are commonly used supplements for the prevention and treatment of osteoporosis. At present, the effective components of the first generation and the second generation calcium supplement products sold in the market are calcium carbonate, calcium lactate or calcium gluconate. Calcium carbonate is insoluble in water and must be neutralized by gastric acid and dissociated into Ca after entering the stomach²⁺Can be absorbed, has strong irritation to stomach, and has bioavailability of about 30-40%. The calcium lactate and the calcium gluconate have good solubility, but low calcium content of 13.0 percent and 9.0 percent, and absorption and utilization rate of about 30 to 40 percent, and the calcium gluconate is not suitable for people with diabetes. The third generation calcium supplement product is amino acid chelated calcium, the bioavailability is greatly improved to about 60-80%, but the absorption and utilization of the calcium supplement product are restricted by amino acid transport carriers.

Disclosure of Invention

The invention aims to provide a sea cucumber peptidation active calcium product and a preparation method thereof, thereby providing a peptidation active calcium supplement preparation with good stability, high calcium absorption rate and bone mineral density improvement to make up for the defects of the existing calcium preparation.

In order to solve the above problems, the present invention provides the following technical solutions:

a preparation method of a sea cucumber peptidation active calcium product comprises the following steps:

1) preparation of sea cucumber bioactive peptide: adding 0.01-0.05% of trypsin into each gram of sea cucumber for enzymolysis, preparing sea cucumber enzymolysis liquid under the conditions that the enzymolysis temperature is 50 ℃, the enzymolysis time is 6 hours and the pH value is 7.0, and obtaining the sea cucumber active peptide by Sephadex G-25 gel column chromatography and spray drying of the enzymolysis liquid;

2) and (3) peptidation treatment: carrying out composite reaction on a calcium chloride solution with the mass ratio of 5% and a sea cucumber bioactive peptide solution with the mass ratio of 10% to form a peptide bioactive calcium solution, wherein the peptide condition is as follows: the mass ratio of the sea cucumber bioactive peptide to the calcium chloride is 3-5: 1, the reaction temperature is 37-50 ℃, and the reaction time is 30-60 min;

3) and (3) dialysis: removing free calcium ions and chloride ions subjected to peptidation in the step 2) by dialysis treatment;

4) and (3) drying: spray drying the solution dialyzed in the step 3), and adding a mannitol protective agent with the mass ratio of 5% before drying;

5) packaging: packaging the dried peptide activated calcium in the step 4) to obtain the sea cucumber peptide activated calcium product.

Further, the peptidation conditions are that the mass ratio of the sea cucumber active peptide to the calcium chloride is 5: 1, the reaction temperature is 50 ℃, and the reaction time is 30 min.

Furthermore, the molecular weight of the sea cucumber active peptide is less than 1000 Da.

The invention also provides a sea cucumber peptidation active calcium product prepared by the preparation method of the sea cucumber peptidation active calcium product.

Advantageous effects

The sea cucumber peptide active calcium has simple preparation process, easy industrialization and mild reaction condition, and is a green processing technology. Researches show that the sea cucumber peptide active calcium obtained by the invention has the characteristic of being easier to absorb than amino acid chelated calcium, does not need an amino acid carrier during operation, is not easy to saturate, does not cause hypercalcemia, has good stability on pepsin and small intestine brush border membrane enzyme and high transport and absorption rate, can realize double-effect simultaneous supplement of peptide and calcium, and is a peptide active calcium supplement preparation with great popularization prospect.

Detailed Description

The present invention is further illustrated below with reference to specific embodiments.